中文摘要：

目的：研究细胞信号传导子和转录激活子6（STAT6）信号传导通路与结肠癌细胞增殖的关系及其可能机制。方法：构建STAT6特异的shRNA慢病毒表达载体,转染人结肠癌细胞HT-29,以阻断STAT6信号通路;用RT-PCR方法检测STAT6 mRNA表达和流式细胞术检测磷酸化STAT6蛋白（pSTAT6）表达来验证构建效果;流式细胞术分析细胞周期变化;MTT法检测肿瘤细胞增殖状况;RT-PCR和Western blot观察p21~（WAF1）、p27~（KIP1）基因表达。结果：成功构建STAT6特异的shRNA慢病毒表达载体;STAT6基因沉默的结肠癌细胞中细胞增殖受到明显抑制（F=62.527,P〈0.001）;STAT6信号通路被阻断的结肠癌细胞中p21~（WAF1）、p27~（KIP1）基因表达明显增多（P〈0.01）。结论：STAT6信号传导通路能促进结肠癌细胞增殖,可能是通过调节p21~（WAF1）、p27~（KIP1）基因的表达起作用。

英文摘要：

Objective： To investigate the relationship between signal transducer and activator of transcription 6 （STAT6） signaling pathway and human colon cancer cells in vitro. Methods： A Lentivirus expression vector of short hairpin RNA （shRNA） specific for STAT6 gene was designed and generated by molecular biological technology. They were transfected into human cancer cells HT-29 to block the STAT6 signaling pathway. The expressions of STAT6 mRNA and phosph- STAT6 protein were detected by RT-PCR method and flow cytometry respectively. The cell cycle condition of the cells in which STAT6 gene expression had been interfered was analyzed by flow cytometry. The cell proliferation was detected by MTT method at the same time. The expressions of p21 WAF1 and p27KIP1 genes were detected by RT-PCR and Western blot, respectively. Results： The lentivirus expression vector of short hairpin RNA （shRNA） specific for STAT6 gene was generated successfully. The cell proliferation ratio in colon cells in which STAT6 gene expression had been interfered was significantly inhibited than that in controlled colon cancer cells （F=62. 527, P〈0. 001）. In the colon cancer cells with STAT6 signaling pathway was blocked, the expression of p21WAF1 and p27KIP1 genes were significantly increased （P〈0.01）. Conclusion： STAT6 signaling pathway could promote the proliferation of colon cancer cells by regulating the expression of p21 WAF1 and p27KIP1 genes.