中文摘要：

人类功能基因RNFl22能够明显抑制细胞生长，导致细胞凋亡．RNFl22含有RING—H2结构域．为了研究RNFl22的RING结构域和凋亡的相互关系，构建了RING结构域突变体．MTT和凋亡实验发现，RNFl22与细胞存活的密切关系依赖于其RING结构域．进一步的实验提示，RNFl22能够负向调节ERK通路，而RING结构域突变型RNFl22则能够增强ERK的磷酸化，提示RNFl22可能通过ERK通路调节细胞的存活．总之，RING结构域对于RNFl22发挥功能起至关重要的作用．

英文摘要：

RNF122 is a novel gene which can inhibit cellular growth and induce apoptosis. RNF122 contains a RING-H2 domain. RING domain mutant was constructed to explore the relationship between RING domain and apoptosis. MTT and apoptosis experiments revealed that RNF122 closely related to cell viability dependent on its RING domain. Further exploration implied that RNF122 can negatively regulate ERK pathway, whereas RING domain mutant RNF122 can enhance the phosphorylation of ERK. It can be concluded that RNF122 may regulate cellular viability through ERK pathway. In short, RING domain is critical to the function of RNF122.