中文摘要：

目的优化大孔吸附树脂分离纯化葡萄叶黄酮类化合物的工艺条件。方法采用分光光度法,以芦丁为标准品,采用硝酸铝显色法测定总黄酮含量;以葡萄叶总黄酮的含量、吸附量和解吸率为考察指标,通过静态及动态实验,筛选出理想的吸附树脂,并通过单因素试验确定了葡萄叶总黄酮富集分离的优选操作条件。结果 D-101树脂是吸附葡萄叶提取液中总黄酮较理想的树脂,对其优化的工艺参数为:最佳吸附p H值:p H=3,洗脱剂浓度:70%的乙醇,最佳上样流速:0.8 m L/min,最佳上样液浓度:65.65 mg/L,最佳解吸流速:0.8m L/min。结论 D-101树脂对葡萄叶总黄酮的吸附量大,解吸容易,环境友好,确定的吸附与洗脱条件简单可行,此工艺具有良好的产业化前景。

英文摘要：

Objective To optimize the separation process for the purification of grape leaf flavonoids by macroporous adsorption resin. Methods The total flavonoids content were determined by aluminium nitrate colorimetric method, taking rutin as the standard. The ideal adsorption of resin has been selected for evaluating the quantity of total flavonoids in grape leaves, the adsorption capacity and desorption rate. The enrichment and separation conditions for the total grape leaf flavonoids were optimized through static and dynamic experiments, and they were evaluated by single factor test. Results D-101 adsorption resin was selected to be the suitable tool for total flavonoids extraction. The optimized parameters were as bellow:pH=3, ethanol 70%, the optimal sample flow rate:0.8 mL/min, sample concentration:65.65 mg/L, desorption velocity:0.8 mL/min. Conclusion The D-101 resin in adsorption the grape leaf total flavonoids is desorption easily, environment friendly and its adsorption and elution conditions are simple and feasible, which illustrates its good prospect for industrialization.