农杆菌是一种能使部分双子叶植物发生冠瘿瘤病害的病原菌,其致病机理是从自身的Ti质粒上转运一段T-DNA到宿主细胞中,并使宿主细胞发生遗传转化。农杆菌是以蛋白质-单链-T-DNA复合物(简称T-复合物)的形式来转运这段T-DNA。T-复合物是通过四型分泌系统(T4SS)转运到宿主细胞中,T-复合物与T4SS之间的识别是由一种被称为T-复合物招募蛋白的蛋白质来完成的。将对农杆菌T-复合物招募蛋白的发现过程和生物学功能鉴定方面的研究进展进行简要评述。
Agrobacterium tumefaciens is a phytopathogenic bacterium that causes the crown gall tumor disease in some dicotyledonous plants by transferring a segment of DNA(T -DNA) from its Ti-plasmid to plant cells and genetically transforming the host cells.Agrobacterium transfers the T-DNA in the form of protein-single-stranded-T-DNA(called T-complex).T-complex is transferred to the host cell through the type IV secretion system(T4SS).The recognition between T-complex and T4SS is fulfilled by a T-complex recruiting protein.This paper will briefly review the discovery of the Agrobacterium T-complex recruiting protein and the progress in identifying the biological function of the Agrobacterium T-complex recruiting protein.