中文摘要：

目的：观察多聚二磷酸腺苷核糖合成酶（PARP）抑制剂对血管紧张素Ⅱ（AngⅡ）刺激的乳鼠心脏成纤维细胞基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制剂-1（TIMP-1）基因转录的影响。方法：新生SD大鼠心脏成纤维细胞原代培养，传代。用PARP抑制剂——3-氨基苯甲酰胺（3-AB）预处理细胞，观察3-AB对AngⅡ诱导心脏成纤维细胞MMP-2、MMP-9及TIMP-1基因表达的影响。结果：AngⅡ刺激心脏成纤维细胞MMP-2、MMP-9及TIMP-1基因表达明显增加，成纤维细胞内PARP活性及PARPl的表达显著增强。使用3-AB抑制PARPl的活性及表达，可以明显降低AngⅡ诱导的心脏成纤维细胞MMP-2、MMP-9及TIMP-1基因表达的增加。结论：PARP在AngⅡ诱导的心脏重构过程中发挥了重要的调控作用。

英文摘要：

Objective：To study the effect of poly（ADP-ribose） polymerase（PARP） inhibitor on cardiac fibroblast treated by angiotensin Ⅱ （Ang Ⅱ ）. Method： Cultured 3rd passage cardiac fibroblasts were incubated with PARP inhibitor 3-aminobenzamide （3-AB） for 30 minutes first and then 0.1μmol/L Ang Ⅱ were added to the culture medium. The incubation time was 24 hours. We explored the reactive oxygen species（ROS）, the PARP activity and the expression of MMP-2, MMP-9 and TIMP-1 in cardiac fibroblast. Result： Ang Ⅱ induced ROS generation,induced PARP activation and PARP1 expression, 3-AB effectively prevented the expression of matrix MMP- 2, MMP-9 and TIMP-1 in cultured fibroblast treated with Ang Ⅱ. Conclusion：Inhibition of PARP activation prevented Ang Ⅱ induced transcriptional expression of MMP-2, MMP-9 and TIMP-1 in cultured fibroblast.