中文摘要：

目的 考察mir-130a-3p及靶基因smad4在肝细胞癌（HCC）组织中表达及相关性,并分析两者表达与肝癌临床病理特征和预后的关系。方法 从石蜡组织包埋的51例肝癌组织及相应癌旁组织中提取总RNA,应用实时荧光定量PCR（qRT-PCR）方法检测mir-130a-3p的表达,并采用免疫组织化学EliVision（IHC）法测定肝癌组织及癌旁组织中smad4表达,分析两者相关性、与临床病理参数及总生存时间（OS）之间的关系。结果 mir-130a-3p在肝癌组织中的表达水平（1.38±0.15）显著低于癌旁组织（2.48±0.16）（P〈0.001）。在肝癌组织中,smad4阳性表达率为72.5%（37/51）,明显高于癌旁组织51.0%（26/51）（P〈0.05）。mir-130a-3p和smad4在肝癌组织中的表达呈负相关性（rs=-0.431,P〈0.05）。mir-130a-3p和smad4的表达水平与肝癌患者TNM分期有关（P〈0.05）,与年龄、性别、有无淋巴结转移、有无脉管癌栓及病理分级无关。肝癌组织中mir-130a-3p高表达患者中位OS（25.6个月）较低表达组（23.1个月）延长,但差异无统计意义;smad4阴性表达组中位OS（26.7个月）较阳性表达组（20.0个月）显著延长（P〈0.05）。结论 mir-130a-3p在肝癌组织中表达下调,可能通过调控smad4的表达参与了肝癌的发生发展,mir-130a-3p有望成为肝癌潜在的治疗靶点及预后判断因子。

英文摘要：

Objective To detect expressions and correlation of mir-13Oa-3p and smad 4 in hepatocellular carcino- ma （HCC）, and analyze the relation of the expressions of both to clinicopathological characters and prognosis of patients with HCC. Methods The total of 51 HCC tissues and 51 adjacent non-tumorous tissues which were ob- tained from the same patients to the HCC samples were collected. Total RNA of all samples were isolated and gene expressions of mir-13Oa-3p were detected using qRT-PCR. Immunohistochemical staining was employed to deter- mine the expression of smad 4 in HCC and adjacent tissues. And then the correlation of mir-13Oa-3p with smad 4, as well as relation of both to clinicopathological characters and over survival of HCC, were analyzed. Results The gene expressions of mir-13Oa-3p in HCC tissues were decreased significantly than that in adjacent non-tumorous tis- sues （1.38 ± 0. 15 vs 2.48 ±0. 16, P 〈 0. 05）. The total of 37 samples had positive expressions of smad 4 out of 51 HCC tissues, with the rate of positive expression of 72.5 %, which were apparently more than 26 out of 51 adja- cent samples, with the rate of positive expression of 50. 0% （P 〈 0. 05）. The level of mir-13Oa-3p was negatively related to that of smad 4 in HCC samples （rn = - 0. 43, P 〈 0. 05 ）. The expressions of both mir-13Oa-3p and smad 4 were related to TNM stage of patients with HCC, however, they had no correlation with age, gender, lymphnode metastasis, cancer embolus of vessel and pathologic grade. The median over survival （OS） of patients with mir-13Oa-3p high expressions was longer than that of patients with low expressions, but it was not significant （25.6 vs 23.1 month）. However, the median OS of patients with smad 4 negative expressions was much longer, compared to that of patients with positive expressions （26. 7 vs 20. 0 month, P 〈 0.05 ）. Conclusion The ex- pressions of mir-13Oa-3p are down-regulated in HCC tissues and maybe participate in oncogenesis and progression of HCC via re