中文摘要：

本文旨在利用粗山羊草的物理图谱及其基因组序列数据库开发普通小麦5DL上Xbarc320-Xwmc215区段与SNP紧密连锁的SSR标记（SNP-SSR）。对AT5D4910-AT5D5010之间的90个SNP位点进行了序列延伸和本地Blast,利用SSR Hunter软件查找SNP位点附近的SSR位点,并利用Primer5设计引物。结果共发现109个SSR位点。对其中的72个位点设计引物,得到了47个5DL上Xbarc320-Xwmc215区段的SNP-SSR标记。对新开发的标记,利用22个小麦品种及人工合成小麦材料、中国春缺体四体及DH群体进行了有效性、多态性的检测及染色体定位。结果表明,这些标记均能扩增出稳定清晰的带型,并且均定位与小麦5D染色体上;其中四个标记Xtdc11、Xtdc31、Xtdc38、Xtdc44整合到了豫麦57与花培3号群体的5DL图谱上。

英文摘要：

The objective of this study was to develop SNP-SSR markers on Xbarc320-Xwmc215 of 5DL chromosome forwheat by using the physical map of Aegilops tauschii and the database of wheat D genome sequence. SSR Hunter softwarewas used to search SSR loci based on SNP sequences and then SNP-SSR primer pairs were designed by using Primer 5.0software. The validity of primer pairs was detected using 22 wheat varieties and synthetic hexaploid wheat materials. Thesemarkers were mapped using DH population and Chinese Spring Nullisomic Tetrasomic lines. 109 SSRs were found among90 SNP extended sequences and local Blast between AT5D4910-AT5D5010. Based on these SSR sequences, 72 SNP-SSRprimer pairs were designed. 47 new SNP-SSR markers on Xbarc320-Xwmc215 of 5DL were obtained. Four of those lociXtdc11, Xtdc31, Xtdc38, and Xtdc44 were integrated into the wheat 5DL genetic map using DH population.