以核DNA ITSl(Internal Transcribed Spacer 1)序列作为分子标记,对我国稻蝗属部分物种种间相互关系进行初步探讨。对获得的7种稻蝗ITSl区序列进行同源性比较、核苷酸使用频率和变异住点统计计算,分析种间核DNA变异,并用芋蝗作为外类群构建NJ和MP分子系统进化树。实验共获得ITSl序列381bp,其中变异位点48个,占所测序列的12.6%,碱基组成分析显示AT含量低于GC含量,与mtDNA富含AT的特征明显不同;核苷酸变异位点统计结果显示,稻蝗属不同种间核苷酸替换数最高为36个,发生在小稻蝗与山稻蝗之间,变异率达11.18%;而无齿稻蝗与短翅稻蝗具有相同的ITSl区序列,彼此之间没有变异。系统发育树显示,小稻蝗和其他稻蝗之间的关系相对较远,是分化较大的种类;日本稻蝗和海南稻蝗与山稻蝗、短翅稻蝗和无齿稻蝗与中华稻蝗关系较近,分别构成一个聚类簇;但是两两种之间的聚类置信度不高,种间关系还有待进一步澄清。
The molecular phylogeny of Oxya species were investigated by sequencing the ribosomal DNA (rDNA) ITS1 sequences. We obtained 381bp sequences in length for the seven Oxya species and one out- group species Gesonula punctifrons. The sequences were compared, the nucleotide frequency calculated, and the molecular phylogenetic trees were constructed by NJ and MP methods using G. punctifrons as out- group. The results showed that: (1) the sequence of ITS1 included 48 variable sites (about 12.60%) and showed higher GC content than AT, which was significantly different from mtDNA sequence with much higher AT preference;(2) the largest nucleotide variation was found between O. intricata and O. agavisa with 36 variable sites(about 11.18%),and no mutation between O. adentata and O. brachyptera; (3) the phylogenetic trees showed that O. hainanensis, O. japonica and O. agavisa was closely clustered; O. brachyptera,O, adentata and O. chinensis have close relationship;O, intricata was obviously remote from other species. In total, the more markers and samples were necessary to further examine the relationship a- mong Oxya species.