中文摘要：

对 Chlamydia trachomatis 的 pORF5 plasmid 蛋白质准备抗体并且开发双抗体三明治的目的为生殖器的 C 的察觉的连接酶的 immunosorbent 试金(DAS-ELISAs ) 。trachomatis 感染。pORF5 蛋白质在 Escherichia coli 被表示并且过去常使 BALB/c 老鼠和新西兰兔子免疫为 DAS-ELISAs 生产 monoclonal 抗体(mAbs ) 和 polyclonal 抗体(pAb ) 的方法。从 186 个尿生殖的感染病人的临床的样品(组我) 并且 62 个健康施主(组 II ) 被在这研究并且由 IDEIA PCE 商业 ELISA 开发的 DAS-ELISAs 在平行检测。二个 hybridoma 房间衬里的结果，命名 2H4 和 4E6，稳定地 secreting 对 pORF5 的特定的 mAbs 被获得。mAb 2H4 被 32 认出(17.20% ，积极识别率) 并且 25 (13.44%) ，在 0 的 mAb 2H4 (0%) 并且(3.22%) 2 从组取样我和 II 分别地。mAbs 2H4 和 4E6 的敏感是 92.11% 和 77.78% ，特性是 100% 并且 96.88% ，分别地在与 IDEIA PCE 商业 ELISA 的关系。为 DAS-ELISAs 的察觉的敏感是 10 ng/mL (基于 2H4 ) 并且 18 ng/mL (基于 4E6 ) 。结论二 DAS-ELISAs 在提供了能为 C 的 serodiagnosis 被认为工具其他的可行、有效的试金的这研究被开发。trachomatis 感染。

英文摘要：

Objective To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis develop double-antibody sandwich enzyme-linked immunosorbent assays （DAS-ELISAs） for detection of genital C. trachomatis infections. and the Methods The pORF5 protein was expressed in Escherichia coil and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies （mAbs） and polyclonal antibody （pAb） for DAS-ELISAs. Clinical samples from 186 urogenital infection patients （groups I） and 62 healthy donors （groups II） were detected in parallel by the DAS-ELISAs developed in this study and by IDEIA PCE commercial ELISA. Results Two hybridoma cell lines, named 2H4 and 4E6, stably secreting specific mAbs against pORF5 were obtained. The mAb 2H4 was recognized by 32 （17.20%, positive recognition rate） and 25 （13.44%）, mAb 2H4 by 0 （0%） and 2 （3.22%） samples from groups I and II, respectively. The sensitivities of mAbs 2H4 and 4E6 were 92.11% and 77.78% and the specificities were 100% and 96.88%, respectively in relation to the IDEIA PCE commercial ELISA. The sensitivities of detection for the DAS-ELISAs were 10 ng/mL （based on 2H4） and 18 ng/mL （based on 4E6）. Conclusion Two DAS-ELISAs were developed in this study that provided a feasible and effective assay that could be considered alternative tools for the serodiagnosis of C. trachomatis infection.