中文摘要：

目的：：通过异种移植的方法制备小鼠子宫平滑肌瘤动物模型。方法：经腹部手术将人子宫肌瘤细胞悬液种植到CB-17 Scid小鼠子宫肌层，手术后缝合腹部切口。定期记录实验小鼠的体重变化及临床症状变化。种植子宫肌瘤细胞后第4周末，观察小鼠子宫大体形态，比较子宫系数和子宫横径；HE染色光镜观察小鼠子宫平滑肌的病理变化。结果：与正常组相比，模型组小鼠的子宫重量增加，子宫系数增大，宫颈和宫体最大直径增大。 HE染色光镜观察显示，与正常组相比，模型组小鼠子宫平滑肌纤维排列紊乱，出现局灶性增生，平滑肌厚度增厚，排列呈卷云状或编织状，界限不清，平滑肌细胞体积增大，细胞核分布密集，呈活跃增生现象，病变与人体子宫肌瘤样增生一致。结论：经腹部手术将人子宫肌瘤细胞悬液种植到CB-17 Scid小鼠子宫肌层的方法能够建立子宫平滑肌瘤小鼠模型。

英文摘要：

Objective：To establish an appropriate animal model of uterine leiomyoma by xenograft. Methods：Human uterine leiomyoma cells suspension were transplanted into the CB-17Scid mice myometrium by abdominal surgery,and sutured the cut. The growth features and histology of the transplanted tumors were observed,and four weeks later,observed the gross morphology of mice,compared uterine coefficient and uterus transverse diameter by the statisti-cal analysis,and the pathological changes of uterine smooth muscle of the mice were observed by the microscope after HE stain. Results：Mouse uterine weight,coefficient,and the maximum diameter of cervical and uterine body increased significantly in model group compared with the normal group. The optical microscope showed the mouse uterine smooth muscle disorder and muscular layer hypertrophy,there were focal hyperplasia,smooth muscle arranged in cirrus-like or braided,the smooth muscle cell volume increased,the cell nucleus densely distributed. That was an active proliferation phenomenon,which was similar to human uterine leiomyoma. Con-clusions：Human uterine leiomyoma cells suspension were transplanted into the CB-17 Scid mouse myometrium by abdominal surgery. This method can establish mouse model of uterine leiomyoma.