中文摘要：

目的探讨邻苯二甲酸二（2-乙基己基）酯（di-2-ethylhexyl phthalate,DEHP）染毒对GC-2 spd细胞半胱氨酰天冬氨酸酶（caspase）蛋白及m RNA表达的影响。方法将体外培养的处于对数生长期的GC-2 spd细胞暴露于含终浓度为0（溶剂对照）、50、100、200μmol/L DEHP的培养基中培养24 h。采用Real-time PCR法检测细胞caspase-3、caspase-8、caspase-9 m RNA的表达,采用Western blotting法检测细胞caspase-3蛋白酶原（procaspase-3）、procaspase-8、procaspase-9的表达。结果与溶剂对照组比较,100μmol/L DEHP染毒组GC-2 spd细胞caspase-3 m RNA的表达水平和200μmol/L DEHP染毒组GC-2 spd细胞caspase-8 m RNA的表达水平及50、200μmol/L DEHP染毒组GC-2 spd细胞caspase-9m RNA的表达水平均较高,差异均有统计学意义（P〈0.05）。与溶剂对照组比较,100、200μmol/L DEHP染毒组GC-2 spd细胞procaspase-3、procaspase-8、procaspase-9的表达水平均较低,差异均有统计学意义（P〈0.05）;且随着DEHP染毒浓度的升高,GC-2 spd细胞procaspase-3、procaspase-8、procaspase-9的表达水平均呈下降趋势。结论 DEHP体外染毒可能通过启动caspase-8和caspase-9进而激活下游caspase-3级联反应,最终诱导生精细胞凋亡。

英文摘要：

Objective To investigate the effects of di-（2-ethylhexyl） phthalate（DEHP） on protein and m RNA expression of caspase in GC-2 Spd cells. Methods GC-2 spd cells were respectively exposed to the DEHP medium with the final concentrations of 0,50,100,200 μmol/L and then cultured for 24 h. The m RNA expression of caspase was measured by Realtime PCR,and the protein expression of procaspase was tested by Western blotting. Results Compared with the control group,the m RNA expression levels of caspase-3 in 100 μmol/L DEHP group,caspase-8 in 200 μmol/L DEHP group,caspase-9 in 50 and 200 μmol/L DEHP group were higher,with statistically significant differences（P〈 0.05）. Compared with the control group,the protein expression of procaspase-3,-8,-9 in 100 and 200 μmol/L DEHP group was lower（P〈 0.05）. With the increase of the DEHP dose,the protein expression of procaspase-3,-8,-9 showed a decreasing trend. Conclusion In vitro exposure to DEHP can induce the apoptosis of GC-2 spd cells through activation of caspase-3 mediated by cleavage of caspase-8 and caspase-9.