中文摘要：

为了对脂多糖应答基因（lrp）的功能进行深入的研究,用镍离子螯合柱（Ni-NTA）纯化后的全长Lrp蛋白免疫新西兰大白兔制备多克隆抗体并吸附去除非特异性反应成分.Western印迹表明,吸附纯化后的抗体可以与Lrp蛋白特异结合,并有较高免疫印迹滴度,为Lrp功能研究提供了重要的工具.激光共聚焦扫描荧光显微镜检测显示Lrp主要位于细胞核膜周围,Western印迹、RT-PCR以及细胞免疫组化染色结果都表明,用LPS刺激后,lrp在人HEK293和U937细胞内的表达均有明显的上升.结果提示,Lrp可能与对Lrp介导的反应有关.

英文摘要：

To examine the lrp gene function further, full-length Lrp protein was purified by Ni-NTA chelating column. Polyclonal antibody was prepared by immunizing rabbit using purified protein, thereafter the antiserum was adsorbed to remove the non-specific reaction components. Westem blot analysis showed that the purified antibody could bind with Lrp protein specifically, and had high titer with immunoblotting. Laser scanning confocal fluorescence microscope observation showed Lrp localized mainly around nucleus membrane. The results of Westem blot, RT-PCR and immunohistochemistry indicated that lrp expression in human HEK293 and U937 cells were both upgraded remarkably by the irritation of LPS, which suggests that Lrp probably correlate with LPS induced response.