中文摘要：

目的 探讨脓毒症大鼠大脑皮质神经元含半胱氨酸的天冬氨酸蛋白水解酶-3（caspase-3）表达的意义.方法 行盲肠结扎穿孔术（CLP）建立脓毒症模型.70只30日龄健康雄性Wistar大鼠,按简单随机法分为CLP组（50只）和对照组（20只）.CLP组于CLP手术后6h、12 h、24 h、48 h,分别取5只大鼠做神经行为学检查,然后处死,取脑组织,采用免疫荧光染色和Western blot法检测大鼠大脑皮质凋亡蛋白caspase-3的表达.对照组不给予CLP,其余处理同CLP组.结果 CLP手术后12 h、24 h和48 h大鼠的神经行为学评分均明显低于对照组（=3.651、3.773、7.155,P均＜0.05）,且随着时间变化,大鼠的神经行为评分逐渐降低,大鼠的整体情况越来越差.免疫荧光检测表明：对照组大鼠大脑皮质中仅表达微量的caspase-3蛋白,而在CLP手术后12 h,脓毒症大鼠大脑皮质中caspase-3蛋白表达明显增加.Western blot检测表明：CLP手术后6h、12 h和24h大鼠大脑皮质caspase-3蛋白表达均明显高于对照组（P均＜0.05）.CLP手术后6h,caspase-3蛋白表达开始升高,12 h达高峰,48 h下降.结论 脓毒症时大鼠的神经行为学评分降低,大脑皮质神经元凋亡蛋白酶caspase-3表达上调.

英文摘要：

Objective To investigate the expression of apoptotic protease cysteinyl aspartate specific proteinase （caspase-3） in cortex neurons of rats with sepsis.Methods Models of rats with sepsis were established by the cecal ligation and puncture （CLP）.Totally 70 cases of 30-day-old male Wistar rats were randomly divided into CLP group （n =50） and control group （n =20）.In CLP group,CLP was performed in the rats.Neurobehavioral score was measured in 5 rats at 6,12,24 and 48 h after CLP surgery,respectively.Then,they were killed and their brains were removed.The immunohistochemical staining and Western blot were used to detect the apoptotic protein caspase-3 expression in cortex of rats.Control group did not undergo CLP,and the other treatment was the same as CLP group.Results Neurobehavioral scores at 12,24 and 48 h after CLP surgery were significantly lower than that in the control group（t =3.651,3.773,7.155,all P 〈 0.05）,and the scores were gradually decreased,overall situation of rats was getting worse along with the time.Caspase-3 protein expressed only in trace amounts in rat cerebral cortex in the control group by immunofluorescence analysis,however,its expression was significantly increased at 12 h after CLP surgery.Western blot test showed that caspase-3 protein expression in rat cerebral cortex at 6,12 and 24 h after CLP surgery was significantly higher than those in control group （all P 〈 0.05）.Its expression began to increase at 6 h after CLP surgery,and reached the peak at 12 h,then decreased at 48 h.Conclusion The neurobehavioral scores decreases and the expression of apeptosis protease caspase-3 increases in cortex of rats with sepsis brain injury.