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中文摘要：
为深入研究小菜蛾对溴虫腈抗性发展机制，采用浸叶法研究溴虫腈对小菜蛾3龄幼虫解毒酶系（多功能氧化酶MFO、酯酶EST和谷胱甘肽孓转移酶GST）活性的影响。结果表明：用溴虫腈处理小菜蛾3龄幼虫48h的致死中质量浓度（LC50）为1．281mg／L；用溴虫腈LC50剂量处理小菜蛾3龄幼虫一定时间后，处理组EST酶活力显著高于对照组，并且随着处理时间的延长，解毒酶活性逐渐升高并保持在较高的水平；MFO酶活力在处理初期就被溴虫腈极显著诱导，但随着处理时间的推移，处理组的MFO酶活力与对照相比差异不显著；而GST酶在处理后则没有被激活。由此可见，溴虫腈处理后能有效的干扰EST和MFO的活性，说明这2种解毒酶可能参与小菜蛾对溴虫腈的代谢过程。
英文摘要：
By using the leaf-dip method,effects of chlorfenapyr on detoxifying enzyme activity in 3rd instar larvae of diamondback moth（DBM）,Plutella xylostella,were investigated.The results showed that LC50 of chlorfenapyr in 48 hwas 1.281mg/L against 3rd instar larvae of diamondback moth;After treatment with sublethal concentrations（LC50）of chlorfenapyr,the activity of esterase（EST）enzyme was significantly higher than the control group,and with the processing time,the enzyme activities gradually increased and maintained at a high level.Mixed-function oxidase（MFO）activity increased significantly after treated for 6h,12 hcompared with those in the control group,but after treated for 24 h,48h,MFO activity was not significantly different from the control.The activity of glutathione S-transferase（GST）enzyme was not activated.In conclusion,chlorfenapyr may induce the activities of EST and MFO detoxifying enzymes in susceptible strains of DBM,esterase and mixed-function oxidase may be involved in the metabolic of the DBM to chlorfenapyr.
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