中文摘要：

目的：研究不同激素组合、激素水平及添加物（水解乳蛋白）对光果甘草6种外植体愈伤组织中总黄酮含量的影响。方法：通过组织培养的方法获得愈伤组织,以芦丁为标准品,采用分光光度法于500 nm处测定愈伤组织中总黄酮的含量。结果：在附加6-BA 4.0 mg/L＋NAA 1.0 mg/L的MS培养基中,节间愈伤组织中总黄酮含量最高,为2.316%±0.015%。培养基中添加水解乳蛋白500 mg/L后极显著地增加了所有外植体愈伤组织中的黄酮含量;在附加了6-BA1.0 mg/L＋NAA1.0 mg/L＋水解乳蛋白500 mg/L的MS培养基中,节间愈伤组织中总黄酮含量最高,为2.376%±0.003%。结论：6-BA与NAA的激素组合,以及水解乳蛋白的添加,均有利于光果甘草愈伤组织总黄酮的生产。

英文摘要：

Objective：To study the effects of hormone kinds,hormone levels and additives（hydrolysate lactoprotein） on content of total flavonoids in 6 callus of Glycyrrhiza glabra L.Method：Callus were obtained by micro-propagation.,the content of flavonoids was determined at wavelength of 510 nm with spectrophotometry by the standard of rutin.Result：The content of flavonoids in callus derived from internodes was the highest among the all treatments,it was as high as 2.316%±0.015% when incubated on MS＋6-BA 4.0 mg/L＋NAA 1.0 mg/L.The contents of flavonoids were significantly increased as hydrolysate lactoprotein was added in MS solid medium.When the internodes were inoculated on MS＋6-BA 1.0 mg/L＋NAA 1.0 mg/L＋ hydrolysate lactoprotein 500 mg/L,the content of flavonoids was the largest as high as 2.376%±0.003%.Conclusion：Combination of 6-BA and NAA,as well as lactoprotein hydrolysate was beneficial to the production of licorice flavonoids.