中文摘要：

本试验旨在研究十二指肠灌注亮氨酸对奶牛胰腺淀粉酶分泌的影响。以4头周岁荷斯坦母牛[（215±7）kg]为试验动物,手术安装十二指肠胰液收集袋、灌注管和回流管以及颈静脉插管,采用4×4拉丁方试验设计,进行十二指肠亮氨酸灌注试验[（灌注水平分别为0、29.5、59.1和88.6μmol/（kg.h）]。结果表明,随亮氨酸灌注水平升高,血浆胰岛素和胆囊收缩素浓度升高（P〈0.05）,胰腺α-淀粉酶合成速率（U/h）呈先升高后降低趋势（P〈0.01）,在29.5μmol/（kg.h）灌注水平时最大。十二指肠灌注亮氨酸也极显著影响了胰腺α-淀粉酶分泌浓度（U/L,P〈0.01;U/g prot,P〈0.01）。结果表明,亮氨酸可能通过刺激胰岛素和胆囊收缩素释放,调控青年奶牛胰腺淀粉酶分泌功能,二者存在剂量效应。

英文摘要：

This experiment was conducted to evaluate the effects of duodenal leucine infusion on pancreatic a- amylase secretion of dairy cows. Four intravenously cannulated yearling Holstein cows [ （215 _ 7 ） kg ] with pancreatic pouch-duodenal reentrant cannulas, duodenal catheters and jugular catheters were used in a 4 x 4 Latin square with duodenal leucine infusions [ 0, 29.5, 59.1 and 88.6 /xmol/（ kg h） ]. The results showed as follows： plasma insulin and cholecystokinin concentrations were increased with leucine infusion level in- creasing （ P 〈 0.05 ）, and pancreatic oL-amylase production rate （U/h） was increased first and then decreased with leucine infusion level increasing （P 〈 0. 01 ）, in which the greatest value was observed at 29.5 p, mol/（ kg h）. Duodenal leucine infusion could also affect a-amylase concentration （ U/L, P 〈 0.01 ; U/g prot, P 〈0.01 ）. These data indicate that leucine can regulate pancreatic a-amylase secretion by stimula- ting the release of insulin and cholecystokinin of young daily cows, and there is a dose-effect relationship be- tween them. [ Chinese Journal of Animal Nutrition, 2013, 25 （ 8 ） ： 1785-1790 ]