中文摘要：

目的：研究人胆管癌细胞株FRH0201中SET和MYND结构域含有蛋白3（SET and MYND domain-containing protein 3,SMYD3）过度表达对DNA甲基化转移酶3B（DNA methyltransferase 3B,DNMT3B）表达及细胞增殖能力的影响。方法：瞬时转染SMYD3真核表达质粒后,RT-PCR检测细胞中DNMT3B mRNA水平的变化;Western blotting检测细胞中DNMT3B蛋白水平的变化;CCK-8检测细胞增殖能力的改变;流式细胞术检测细胞周期的改变。结果：以未处理组为对照,胆管癌细胞FRH0201在转染pEGFP-C3-SMYD3质粒后,DNMT3B蛋白及mRNA表达均显著上升（P〈0.01）;细胞的增殖能力显著提高、细胞增殖速度加快（P〈0.05）;进入G_2/M期的细胞明显增多（P〈0.05）。结论：过度表达SMYD3,可引起细胞中DNMT3B的表达上调并增强细胞增殖能力。

英文摘要：

AIM：To explore the effect of SET and MYND domain-containing protein 3（SMYD3 ） over-expression on the expression of DNA methyltransferase 3B（DNMT3B） and the proliferation ability in human cholangiocarcinoma cell line FRH0201.METHODS：Transient transfection of SMYD3 eukaryotic expression plasmid pEGFP-C3-SMYD3 into human cholangiocarcinoma cell line FRH0201 was performed.The expression of DNMT3B at mRNA and protein levels was detected by RT-PCR and Western blotting,respectively.Cell proliferation was examined by CCK-8 method and cell cycle situation was checked by flow cytometry.RESULTS：After transfected with SMYD3 eukaryotic expression plasmid pEGFP-C3-SMYD3,the over-expression of SMYD3 in FRH0201 cells was observed.Compared with the untransfected cells,the expression of DNMT3B was significantly increased（P〈0.01）,the proliferation rate was obviously accelerated（P〈0.05） and the number of the cells in G_2/M phase was significantly increased（P〈0.05） in FRH0201 cells transiendy transfected with pEGFP-C3-SMYD3 plasmid.CONCLUSION：The transient transfection of pEGFPC3-SMYD3 plasmid induces over-expression of DNMT3B and promotes the proliferation of human cholangiocarcinoma cell line FRH0201.