中文摘要：

目的：研究心肌梗死患者血清中miR-148b表达量与心肌损伤、心肌纤维化的相关性。方法：选择2013年3月-2016年10月期间在汉中市中心医院诊断为急性心肌梗死（AMI）的130例患者以及体检的100例健康者,分别作为AMI组和对照组,采集血清并采用荧光定量PCR试剂盒检测miR-148b的表达量,采用酶联免疫吸附试剂盒检测心肌损伤标志物、心肌纤维化标志物的含量。结果：AMI组患者血清中miR-148b的表达量以及肌酸激酶同工酶（CK-MB）、肌钙蛋白T（cTnT）、心脏型脂肪酸结合蛋白（H-FABP）、Ⅰ型前胶原羧基端肽（PICP）、Ⅲ型前胶原氨基端肽（PⅢNP）、CTX-I、转化生长因子（TGF）-β1、GDF-15的含量显著高于对照组（P〈0.05）;miR-148b高表达AMI患者血清中CKMB、cTnT、H-FABP、PICP、PⅢNP、CTX-I、TGF-β1、GDF-15的含量显著高于miR-148b低表达的AMI患者（P〈0.05）。结论：心肌梗死患者血清中高表达的miR-148b对心肌损伤、心肌纤维化具有促进作用。

英文摘要：

Objective：To study the correlation of serum miR-148 bexpression with myocardial injury and myocardial fibrosis in patients with myocardial infarction.Methods：A total of 130 patients who were diagnosed with acute myocardial infarction and 100 healthy subjects who received physical examination in Hanzhong Central Hospital between March 2013 and October 2016 were selected and enrolled in AMI group and control group respectively.Serum was collected,fluorescent quantitative PCR kit was used to detect miR-148 bexpression,and enzyme-linked immunosorbent assay kit was used to detect the contents of myocardial injury markers and myocardial fibrosis markers.Results：Serum miR-148 bexpression as well as CKMB,cTnT,H-FABP,PICP,PIIINP,CTX-I,TGF-β1and GDF-15 levels in AMI group was significantly higher than those in control group（P〈0.05）;serum CK-MB,cTnT,H-FABP,PICP,PIIINP,CTX-I,TGF-β1and GDF-15 levels in AMI patients with high miR-148 bexpression were significantly higher than those in AMI patients with low miR-148bexpression（P〈0.05）.Conclusion：Highly expressed miR-148 bin serum of patients with myocardial infarction can promote myocardial injury and myocardial fibrosis.