中文摘要：

应用偏最小二乘（PLS）算法对同步荧光光谱严重重叠的诺氟沙星、盐酸洛美沙星和乳酸左氧氟沙星药物三组分混合体系进行波谱解析，建立了该混合体系含量同时测定的新方法。在pH2．87B-R缓冲溶液中，波长差△λ=190nm时，诺氟沙星、盐酸洛美沙星和乳酸左氧氟沙星的测量线性范同分别为0．016～0.40μg·mL^-1，0．01～0．336μg·mL^-1和0．01～0．336μg·mL^-1；检出限分别为0．0126，0．006和0．0072μg·mL^-1。采用PLS法结合同步荧光法对鳗鱼样品进行测定，结果令人满意。

英文摘要：

In the present paper, the synchronous fluorimetric spectra of norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate with Δλ= 190 nm in a B-R buffer medium of pH 2.87 were measured, and the partial least squares （PLS） method was applied to the quantitative resolution of the seriously overlapped fluorimetric spectra of these compounds. The linear ranges for norfloxacin, lomefloxacin chlorhydric acid, and levofloxacin lactate are 0.016-0.40 μg·mL^-1, 0.01-0.336μg·mL^-1 and 0.01-0.336 μg·mL^-1 , respectively. The limits of detection are 0.012 6 μg·mL^-1 for norfloxacin, 0.006 μg· mL^-1 for lomefloxacin chlorhydric acid, and 0.007 2 μg·mL^-1 for levofloxacin lactate. The analytical results by PLS were compared with principal components regression （PCR） and classical least squares （CLS）, and this method was applied to the determination of these three compounds in eel samples with satisfactory results.