中文摘要：

瞄准：学习在 cyclooxygenase (艇长) 之间的关系食道的有鳞的癌 EC109 房间的 -2 基因和增长和 apoptosis。方法：RNA 干扰(RNAi ) 和房间 transfection 的技术，以及在裸体老鼠的 oncogenicity 的层次，被用来在食道的有鳞的癌房间(ESCC ) 学习 COX-2 的角色线 EC109。后面的 RNAi 和 transfection，西方的弄污分析被用来决定 COX-2 蛋白质的表示。3-(4,5-dimethylthiazol-2-yl )-2,5-diphenyl-tetrazolium 溴化物(MTT ) 减小试金被用来评估细胞生长，并且流动 cytometry 被用来检测细胞 apoptosis。结果：西方的弄污分析证明那 COX-2 表情显著地在与 COX-2-specific 对待的 EC109 房间被减少短介入 RNA (siRNA ) 但是与 COX-2 在 EC109 房间 transfected 被增加。而且， COX-2 siRNA 处理禁止了房间增长(P < 0.01 ) 并且分别地在 EC109 房间导致了 apoptosis，由 MTT 试金并且由流动 cytometry 决定了。相反，导致的 transfected COX-2 增加了房间增长(P < 0.05 ) 并且在 EC109 房间的减少的 apoptosis。另外，有 COX-2 siRNA 和阿司匹林的房间的联合处理有 synergistic 效果(P < 0.01 ) 。为测量 tumorigenicity 的实验，更大的体积和重量的异种皮移植肿瘤与另外的组相比在 COX-2 组被发现(P < 0.05 ) 。阿司匹林的大剂量有效地在裸体老鼠禁止了肿瘤生长(P < 0.05 ) ，并且肿瘤抑制的率在高剂量的阿司匹林组是 51.8% 。结论：COX-2 在 ESCC carcinogenesis 起一个很关键的作用，并且与阿司匹林相结合的 COX-2 siRNA 有潜力是为 ESCC 的处理的 anticancer 治疗。

英文摘要：

AIM：To study the relationship between the cyclooxy-genase（COX）-2 gene and the proliferation and apopto-sis of esophageal squamous carcinoma EC109 cells.METHODS：The techniques of RNA interference（RNAi）and cell transfection,as well as the levels of oncogenic-ity in nude mice,were used to study the role of COX-2 in the esophageal squamous carcinoma cell（ESCC）line EC109.Following RNAi and transfection,Western blot-ting analysis was used to determine the expression of the COX-2 protein.The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium bromide（MTT）reduction assay was used to evaluate cell growth,and flow cytometry was used to detect cell apoptosis.RESULTS：Western blotting analysis demonstrated that COX-2 expression was significantly reduced in EC109 cells treated with COX-2-specif ic short interfering RNA（siRNA）but was increased in EC109 cells transfected with COX-2.Furthermore,COX-2 siRNA treatment in-hibited cell proliferation（P 〈 0.01）and induced apop-tosis in EC109 cells,as determined by an MTT assay and by flow cytometry,respectively.In contrast,trans-fected COX-2 led to increased cell proliferation（P 〈 0.05）and decreased apoptosis in EC109 cells.In addition,combination treatment of cells with COX-2 siRNA and aspirin had a synergistic effect（P 〈 0.01）.For experi-ments measuring tumorigenicity,xenograft tumors of a greater volume and weight were found in the COX-2 group compared with other groups（P 〈 0.05）.A large dose of aspirin inhibited tumor growth in nude mice ef-fectively（P 〈 0.05）,and the rate of tumor suppression was 51.8% in the high-dose aspirin group.CONCLUSION：COX-2 plays a very critical role in ESCC carcinogenesis,and COX-2 siRNA combined with aspirin has the potential to be an anticancer therapy for the treatment of ESCC.