中文摘要：

目的：动态观察N-苄基氟哌啶醇氯化物（F3）对大鼠胸主动脉平滑肌细胞内Ca^2＋的影响。方法：用Ca^2＋荧光染料Fluo-3-AN负载大鼠胸主动脉平滑肌细胞，在激光扫描共聚焦显微镜上测定细胞内游离钙的变化。结果：在含CaCl2（1．26mmol／L）的细胞外液中，30mmol／L KCl使细胞内钙荧光强度（FI）迅速增强，F3可以拮抗KCl诱导钙兀增强作用，并呈量效依赖和时间依赖关系。4种浓度F3（0．01、0．1、1．0、10μmaol／L）作用3min时，使KCl诱导细胞内钙兀从（81±4）％分别降至（71±18）％（n=20，P〈0．05）、（51±12）％（n=20，P〈0．01）、（39±14）％（n=20，P〈0．01）、（28±14）％（n=20，P〈0．01）。钙FI变化最快的时程是在给药后的0-30s。结论：F3通过阻断细胞膜电压依赖性钙通道降低平滑肌细胞内钙浓度，这可能是其扩张血管，改善心肌缺血的机制。

英文摘要：

Objective： To investigate the effects of N-benzyl haloperidol chloride（ F3 ） on intracellular calcium（ [ Ca^2＋] ） in vascular smooth muscle cells（VSMCs） isolated from thoracic aortas of rat. Methods： VSMCs were loaded with a calcium sensitive fluorescent dye Fluo-3-AM, and [Ca^2＋]; was determined by the use of laser scanning confocal microscope. Results： In the extraceUular solution with Ca^2＋ at the concentration of 1.26 mmol/L, 30 mmol/L KCI induced a rapid increase of intracellular calcium fluorescence intensity（FI）. F3 antagonized the increase of FI induced by KCI in both concentration dependent manner and time dependent manner. After 3 minutes of the administration of F3 at the concentration of 0.01, 0. 1, 1.0, 10μmaol/L, the increased FI of the VSMCs induced by KCI was decreased from（81±4）% to（71 ± 18）%（n=20, P〈0.05）, （51±12）%（n=20, P〈0.01）, （39±14）%（n=20, P〈0.01）, （28±14）%（n = 20, P 〈 0.01）, respectively. During 0-30 seconds after the administration of drugs, the changes of FI were more rapid. Conclusion： F3 reduces[Ca^2＋]; via inhibition of voltage dependent calcium channel, which is possibly involved in the mechanism of its vasodilatory and anti-myocardial ischemic effects.