中文摘要：

目的：观察全蝎蜈蚣对胶原免疫性关节炎（Collagen-InducedArthritis，CIA）大鼠外周血CIM＋CD25＋FoxP3＋Treg细胞的调节作用。方法：Wistar大鼠60只，随机分成6组，分别为正常对照组、模型对照组、全蝎蜈蚣高（0．4g／kg）、中（0．2g／kg）、低剂量（0．1g／kg）组和Ⅱ型胶原蛋白（60μg/kg治疗组（CⅡ组），二型胶原蛋白诱导大鼠关节炎，并采用流式细胞术检测大鼠外周血CD4＋CD25＋FoxP3＋Treg细胞及酶联免疫吸附试验检测血清中IL-2表达水平。结果：与模型组比较，全蝎蜈蚣高、低剂量组大鼠外周血CD4＋CD25＋T细胞及CD4＋CD25＋FoxP3＋Treg细胞水平显著升高，且中、低剂量组血清IL-2水平显著下降。结论：全蝎蜈蚣治疗关节炎大鼠可能是通过上调外周CIM＋CD25＋F0xP3＋Treg细胞表达水平以恢复或重建免疫耐受而实现的。

英文摘要：

Objective ：To study the regulation effect of Scorpio and Scolopendra on CD4＋ CD25＋ FoxP3＋ Treg Cell in peripheral blood from rats with collagen-induced arthritis. Methods：Sixty female Wistar rats were randomly divided into 6 groups, normal control group, model control group, low-dose, middle-dose and high-dose Scorpio and Scolopendra group, and the typeⅡ collagen group. Rats＇ arthritis was induced by collagen. The number of CD4＋ CD25＋ FoxP3＋ Treg cell in peripheral blood was tested by flow cytometry, and the level of IL-2 in serum was detected by enzyme linked immunosorbent assay. Results：Compared with the model groups, the levels of CD4＋ CD25＋ Treg cell and CD4 ＋ CD25＋ FoxP3＋ Treg cell were increased obviously in the high and low dose of Scorpio and Scolopendragroups （P 〈0. 05 or P 〈0.01 ） ,while the level of IL-2 in serum was decreased remarkably in the middle and low dose of Scorpio and Scolopendra groups （P 〈0.05 or P 〈0. 01 ）. Conclusion：It is realized that Scorpio and Scolopendra effectively treat RA by regulating the level of CIM＋ CD25＋ FoxP3＋ Treg cell to restore immunological tolerance.