中文摘要：

基于生物质资源生产环境友好的生物燃料，对经济和社会的可持续发展具有重要意义，但其生产成本高的问题十分突出，而高效生产菌株的获得是解决这一问题的根本出路。以下综述了利用系统生物学研究所获得的信息进行菌种改造的过程，重点论述了生产菌株胁迫耐受性方面的研究进展，并讨论了系统生物学、合成生物学和代谢工程技术在改造生物燃料生产菌株中的应用，展望了合成生物学在构建高效生物能源生产菌株方面应用的前景。

英文摘要：

Biofuels are renewable and environmentally friendly, but high production cost makes them economically not competitive, and the development of robust strains is thus one of the prerequisites. In this article, strain improvement studies based on the information from systems biology studies are reviewed, with a focus on their applications on stress tolerance improvement. Furthermore, the contribution of systems biology, synthetic biology and metabolic engineering in strain development for biofuel production is discussed, with an expectation for developing more robust strains for biofuel production.