中文摘要：

目的：探索G蛋白偶联受体激酶结合蛋白1（G protein coupled receptor kinase interacting protein 1,GIT1）的RNA发夹结构（hairpin）（GIT1-RNAh）对成骨细胞内肌球蛋白轻链（myosin light chain,MLC）的分布及磷酸化的影响,并分析其机制. 方法：取培养至第6代的鼠成骨细胞,随机分成两组,分别用包含GIT1-RNAh（实验组）和绿色荧光蛋白（green fluoresence protein,GFP）的发夹结构（GFP-RNAh）的腺病毒（对照组）感染12 h,再将每组分成有.无血小板衍生生长因子（platelet-drived growth factor,PDGF）刺激的两组,免疫荧光染色方法检测成骨细胞内MLC的位置,Western blot检测MLC的磷酸化.结果：免疫组织化学观察：和对照组相比,实验组明显扰乱了成骨细胞MLC的分布;Western blot观察：和对照组相比,实验组明显抑制了MLC的磷酸化（P 〈 0.05）.结论：GIT1的发卡结构过表达可干扰MLC的分布和其磷酸化.

英文摘要：

Objective：To study the effect of G protein coupled receptor kinase interacting protein 1（GIT1） RNA hairpin （GIT1-RNAh） on Myosin light chain（MLC） distribution and phosphorylation in osteoblasts. Methods：The sixth passage osteoblasts were divided into two groups infected with GIT1-RNAh （experimental group） and Green Fluoresence protein RNA hairpin （GFP-RNAh） （control group） adenovirus for 12 hours respectively. Each group was further classfied into two groups according to PDGF stimulation or not. MLC distribution and phosphorylation were analysized by immunoflurescence stain and western blot respectively. Results：Immunoflurescence stain results showed that the GIT1-RNAh significantly interfered with MLC distribution. Western Blot results showed that MLC phosporylation was dramatically inhibited in osteoblasts infected with GIT1-RNAh adenovirus after PGDF stimulation （P 〈 0.05）. Conclusion：GIT1-RNAh overexpression interfered with MLC distribution and decreased MLC phosphorylation.