Homeodomain 白氨酸拉链(HD 压缩) 蛋白质是涉及植物开发的 transcriptional 因素。在这研究,一 cDNA 克隆(Gossypium hirsutum homeobox1,指明的 GhHB1 ) 编码 HD 压缩蛋白质从一个棉花根 cDNA 图书馆被孤立。GhHB1 cDNA 是在长度的 1132 bp,包括 828 bp 与 275 氨基酸编码肽的开的读框架,和 5'-/3'-untranslated 区域。包含 homeodomain 和一个充满白氨酸的拉链主题的预言的 GhHB1 蛋白质与另外的植物 HD 压缩蛋白质分享相对高的身份。用量的即时 RT-PCR 的分析显示 GhHB1 基因主要在根和胚轴被表示。而且, GhHB1 抄本大部分在早根开发被积累,并且显著地减少了,建议基因可能在根的早开发工作很,根推进的底层发展了。在有 1% NaCl 的处理下面, GhHB1 基因的表示水平戏剧性地在根被增加。同样,在根的 GhHB1 活动由 abscisic 是起来调整的。这些结果暗示 GhHB1 可能响应盐应力并且到 abscisic 发信号起一个重要作用。
Homeodomain-leucine zipper (HD-Zip) proteins are transcriptional factors involved in plant development. In this study, one cDNA clone (Gossypium hirsutum homeoboxl, designated GhHB1) encoding HD-Zip protein was isolated from a cotton root cDNA library. The GhHB1 cDNA is 1132 bp in length, including an 828 bp open reading frame that encodes a peptide with 275 amino acids, and 5'-/3'- untranslated regions. The predicted GhHB1 protein containing a homeodomaln and a leucine-rich zipper motif shares relatively high identity with other plant HD-Zip proteins. Analysis using quantitative real-time RT-PCR indicated that the GhHB1 gene is predominantly expressed in roots and hypocotyls. Furthermore, GhHB1 transcripts were largely accumulated in early root development, and significantly reduced to very low levels as roots further developed, suggesting that the gene might function in the early development of roots. Under treatment with 1% NaCI, the expression level of the GhHB1 gene was dramatically increased in roots. Likewise, GhHB1 activity in roots was up-regulated by abscisic acid. These results imply that GhHB 1 might play an important role in response to salt stress and to abscisic acid signaling.