中文摘要：

以红松（Pinus koraiensis）木质部和韧皮部为研究材料，从提取方法、粉碎程度、提取次数、色谱条件以及重现性、精密度、回收率等方面对木质化器官糖类——果糖、葡萄糖和蔗糖的样品制备方法进行了研究。超声法较水浴法能使韧皮部3种可溶性糖提取率提高2％-15％（P〉0．01），但是可以使木质部可溶性糖的提取率提高57％～73％（P〈0．01）。韧皮部和木质部样品可溶性糖的提取率随着提取次数的提高均呈现显著升高的趋势，LangrnuirEXT1能够很好地模拟这种关系，在保证3种糖类均达到90％的精度下，木质部和韧皮部的提取次数需要至少3次。样品粉碎程度对木质部和韧皮部糖类提取效果表现不同：木质部样品的粉碎粒径为0．15mm时，3种糖类的提取效果最高；韧皮部样品粉碎得越细，可溶性糖的提取效果越好，指数模型模拟效果很好（r^2〉0．9）。

英文摘要：

An experiment was conducted to study the preparation methods of fructose, glucose and sucrose in phloem and xylem of Pinus koraiensis in terms of extraction method, size of ground sample, times of extraction, as well as HPLC conditions including repeatability, precision and recovery rate. The extraction rate of fructose, glucose and sucrose from phloem samples for ultrasonic extraction method showed 2% - 15% higher than that for hot water extraction method （p 〉 0.01 ）. However, in the case of xylem samples, the extraction rate of ultrasonic extraction method was 57 % -73% higher than that of hot water extraction method （p 〈 0.01 ）. Fructose, glucose and sucrose from phloem and xylem all showed a significant increase with extraction times, and the LangmuirEXT1 model could well simulate this trend. Under the precondition of extracting 90% of the theoretical maximum values of fructose, glucose and sucrose, the xylem and phloem samples should be extracted at least 3 times according to the results. The degree of grinding for the samples had various effects on the extraction rates of fructose, glucose and sucrose, and the extraction effects of fructose, glucose and sucrose were the best when the particle size of the xylem samples was 0.15 mm, while the extraction effect of soluble sugar was better when the particle size of the phloem samples was small enough. Exponential model showed the best simulation effect.