中文摘要：

目的：明确GDNF启动子I区在人脑胶质瘤中H，赖氨酸残基9位乙酰化（H3K9Ac）情况，探讨其对于GDNF在胶质瘤中表达的影响。方法：RT-PCR检测各组中GDNFmRNA的表达；建立基于Real．timePCR分析的染色质免疫共沉淀（CHIP）方法，检测12例胶质瘤与6例正常脑组织中GDNF基因启动子I区王H3组蛋白乙酰化情况。结果：Real-timePCR验证人脑胶质瘤GDNFmRNA的表达，转录水平随级另q的增高而增高，且低级别组、高级别组与正常组之间存在显著的统计学差异（P〈0．05）。启动子I区的H，组蛋白乙酰化水平，正常组与低级别组和高级别组之间比较均有显著性差异（P〈0．05），且低级别与高级别之间也有显著性差异。结论：在人脑胶质瘤组织中，GDNF启动子I区发生了H3组蛋白高乙酰化修饰，这种修饰很可能会影响GDNF基因的表达。

英文摘要：

Objective： To observe the histoneacetylation of GDNF promoter 1 region in human glioma, in order to explore the effect of methylation on the expression of GDNF in glioma. Methods： RT - PCR detection in each group GDNF mRNA expression; Based on the analysis of the Real - time PCR chromatin immune coprecipitation （CHIP） method, testing 12 cases of gliomas and 6 cases of normal brain tissue GDNF gene promoter region H3 histone acetylation situation. Results： Real - time PCR test the brain glioma GDNF mRNA expression shows that transcription level with the level of higher and higher. The low level group, high level group and normal group there was significant statistical difference （P〈0.05）. The promoter I H3 histone acetylation level, there was significant difference （P〈0.05） among the normal group, low level group and high level group, and there was also significant difference between low level and high level, Conclusion： Acetylation occuerred in GDNF promoter I region might influence the expression of GDNF in glioma