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中文摘要：
以木贼为材料，研究木贼的提取物对蘑菇酪氨酸酶的效应．结果表明，木贼的乙醇提取物对蘑菇酪氨酸酶有较强的抑制作用，其IC50值为0．375mg／mL抑制机理为可逆混合型．DPPH实验显示木贼的乙醇提取物具有较强的抗氧化作用，说明木贼乙醇提取物通过清除氧自由基来抑致黑色素生长的作用．
英文摘要：
Tyrosinase is an enzyme that is very important in the browning of fruit and vegetable. Browning of animal usually is caused by effect of Tyrosinase itself. The extracts from equisetum is separated to get the coarse inhibitor of tyrosinase by pounding to pieces, filtrating, extracting, distilling to concentrate liquid attained. We use this inhibitor act with mushroom tyrosinase. Through this way, we can detect the influence of coarse inhibitor on mushroom tyrosinase ： The inhibitor＇s concentration leading to 50%. activity lost（ IC50） of it were determined to be 0. 375 mg/mL. The mechanism of this coarse extraction effecting on enzyme is reversible. The type of this coarse extraction effecting on enzyme is mixed type. The inhibition constant （ K1 ） was determined to be 0.472 mg/mL. The KIS was determined to be 1. 201 mg/ mL.
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