中文摘要：

目的研究HBeAg阳性慢性乙型肝炎患者HBV变异特点。方法PCR扩增并克隆HBeAg阳性慢性乙型肝炎患者血清中HBV全基因组DNA，测序并进行基因结构分析。结果获得23株HBV全基因组DNA，它们均属于c或B基因型。与中国HBVB、C基因型参照序列相比，HBeAg阳性慢性乙型肝炎患者来源的HBV在表面抗原、P蛋白、X蛋白的反式激活区及增强子II／核心启动子区发生了一些有意义的共有变异。结论HBV变异可能与HBeAg阳性慢性乙型肝炎的发生、发展有关。

英文摘要：

To study the characteristics of full-length Hepatitis B Virus （HBV） genomes isolated from HBeAg positive chronic hepatitis B（CHB） patients. Amplified the full-length HBV genomes by PCR from the serum of HBeAg positive CHB patients, sequenced and analyzed the structure of HBV genomes. Obtained twenty-three full-length HBV DNAs from the dif- ferent CHB patients. Phylogenetic analysis revealed that all HBV strains could be categorized into genotype B or C. Structural analysis showed that, as compared to standard strains of Chinese HBV B,C subtypes, HBV obtained shared meaningful consen- sus mutations in B/T cell epitopes of surface and P protein, transactive domain of X protein and enhancer II/core promoter re- gions. Genotvpe and gene mutation of HBV may closely correlated with the carcinogenesis of HBV-related chronic hepatitis B.