中文摘要：

为减少样品的污染，简化样品提纯的步骤，提高细胞破碎率，设计并制作了一种低电压微型装置实现了细胞破碎。根据细胞破碎原理，设计了梳状微型装置电极模型，利用计算流体力学（Computational Fluid Dynamics，CFD）分析软件，对微型装置电极产生的电场进行模拟分析，运用了剥离工艺的标准光刻胶处理方法，在玻璃基底上制备了6对Ti／Pt微电极。采用幼仓鼠肾细胞（BHK21）对微型装置进行了实验测试。实验表明，BHK21细胞破碎的最佳电压为3V。该装置采用微细加工技术，功耗低、体积小，低电压实现细胞破碎。

英文摘要：

A low voltage cells lysis miniature device is introduced in this thesis. In order to reduce the pollution of samples, simplify the sample purifying steps and improve the cell crushing rates, a new device for crushing cells with low voltage is designed and made in this thesis. According to the cell crushing principles, a paralleled miniaturized module is made. Imitating and analyzing chips＇ electric field with CFD software,researchers have made six pairs of electrodes on the glass base with lithography of lift-off. An experiment with BHK21 is done to test the chip. The experiment shows that the most suitable transmembrane voltage is 3 V. This device is made with microfabrication technology. It has advantages of low energy consuming, small bulk and crushing cells under low voltage.