非编码的 RNA 基因是否在性决心系统的繁殖生物学和进化起一个重要作用,是一个重要问题。我们在果蝇种类之中报导性相关的非编码的 RNA (ncRNA ) 基因的鉴定和 ncRNAs 表示模式的分析。我们检测了被 RT-PCR 的一条综合途径和顺序保存的计算分析越过种类在 D.melanogaster 的性腺表示的 12 候选人 ncRNAs。我们试验性地在密切相关的种类 D 的头,卵巢和睾丸分析了这些 ncRNA 基因抄本。simulans, D。yakuba, D。pseudoobscura 和 D。virilis。在密切相关的种类之中的大多数 ncRNA 碎片的表示的出现和程度看重要分叉的 Weobserved。
Whether or not non-coding RNA genes play a significant role in reproductive biology and evolution of sex determination systems is an important problem. We report identification of sex-related non-coding RNA (ncRNA) genes and an analysis of ncRNAs expression patterns among Drosophila species. We detected 12 candidate ncRNAs that are expressed in the gonads of D. melanogaster by an integrative approach of RT-PCR and computational analysis of sequence conservation across species. We experimentally analyzed these ncRNA gene transcripts in head, ovary and testis of closely related species D. simulans, D. yakuba, D. pseudoobscura and D. virilis. We observed that the occurrence and extent of expression of most ncRNA fragments among closely related species show significant divergence.