中文摘要：

尿路上皮癌抗原1（UCA1）是一种长链非编码RNA,在多种肿瘤内高表达.然而,其在宫颈癌细胞和组织中的表达报告颇不一致,且功能尚未确定.本文探索UCA1在宫颈癌He La细胞中的生物学功能.实时定量PCR（qRT-PCR）结果显示,UCA1、p21和p53 mRNA在阿霉素（doxorubicin,DOX）或γ射线照射的He La细胞中表达上调;相反,敲减p53表达则可抑制DOX诱导的UCA1上调.表明DNA损伤诱导的UCA1可能与p53有关.转染结合CCK8检测He La细胞增殖活力结果显示,与对照比较,过表达UCA1促进He La细胞增殖,干扰UCA1表达则减缓细胞增殖.此外,流式细胞术结果显示,过表达UCA1导致阿霉素诱导的凋亡率下降;siRNA抑制UCA1表达后引起细胞G2/M期比例上升,S期下降,且阿霉素诱导的细胞凋亡率上升.上述结果说明,DNA损伤诱导的UCA1可促进He La细胞增殖,减少细胞凋亡.然而,是否DNA损伤诱导的UCA1上调依赖p53尚需进一步实验证明.

英文摘要：

Urothelial carcinoma antigen 1（ UCA1） is a long non-coding RNA that highly expressed in tumors. The expression of UCA1 in cervical cancers is debated for its biological functions. We explore the function of UCA1 in He La cells. Results of qRT-PCR showed that UCA1,p21 and p53 were upregulated when He La cells were treated with doxorubicin（ DOX） or γ-irradiation; And siRNA-mediated suppression of p53 reduced the DOX-induced UCA1 up-regulation. CCK8 assay was used to evaluate the effect of UCA1 on He La cells proliferation. The result showed that,compared with control group,overexpression of UCA1 promoted cell proliferation; repression of UCA1 by siRNA inhibited cell proliferation. In addition,flow cytometry analysis revealed that ectopic expression of UCA1 inhibited DOX-induced cell apoptosis; repression of UCA1 by siRNA resulted in G2/ M phase arrest and cell apoptosis. These data suggested that DNA damage-induced UCA1 upregulation promoted He La cellproliferation and protected cells from apoptosis.