中文摘要：

目的：研究创伤后应激障碍（PTSD）大鼠杏仁核神经元细胞凋亡及三偏磷酸酶（TMP）活性的变化。方法：成年健康雄性Wistar大鼠50只，随机分为连续单一应激（SPS）模型的1、4、7、14d组及正常对照组。应用Annexin V-FITC／PI双标记流式细胞技术定量检测及分析PTSD大鼠杏仁核神经元细胞凋亡率；利用透射电镜观察杏仁核神经元超微结构改变；利用酶组织化学方法检测杏仁核神经元TMP酶活性的变化。结果：SPS后，杏仁核神经元发生了凋亡的特征性形态学改变，杏仁核神经元细胞凋亡率显著增加，TMP酶活性明显比正常对照组增强，均于第4日达高峰。7d和14d凋亡率逐渐减少、TMP酶活性减弱。结论：PTSD大鼠杏仁核神经元发生细胞凋亡，凋亡率增加的同时TMP酶活性相应增强，提示TMP酶参与杏仁核神经元凋亡产物的吞噬。

英文摘要：

Objective： To study the apoptosis and variation of thiamine monophosphatase （TMP） activity in arnygdala neurons of posttraumatic stress disorder （PTSD） rats. Methods： A total of 50 healthy, male Wistar rats were randomly divided into 1, 4, 7 and 14 d groups of single prolonged stress （SPS） and a normal control group. Annexin V-FITC/PI double-labeled flow cytometry was applied to quantitatively detect and analyze the apoptosis rate in amygdala neurons of PTSD rats; A transmission electron microscopy was used to observe the change of ultrastructure in the amygdala neurons; and enzymohistochemistry was used to detect the variation of TMP activity in amygdala neurons. Results： After SPS, the amygdala neu rons had characteristic morphologic changes of apoptosis; apoptotic rate of amygdala neuron was significantly increased and TMP activity was significantly enhanced compared with that of the normal control group. Both of them reached the peak at 4 d. At 7 d and 14 d, the apoptotic rate was gradually decreased and TMP activity was weakened. Conclusion： Amygdala neu rons of PTSD rats have apoptosis. The apoptotic rate increased, and the TMP activity enhanced simultaneously, which indicate that the TMP is involved in neuronal apoptosis products phagocytosis in amygdala.