中文摘要：

目的探讨人参皂甙Rd及人参皂甙Rd与川芎嗪和（或）葛根素联合应用的脑保护效应，并比较单独使用人参皂甙Rd与联合用药之间保护效应的差异。方法60只雄性SD大鼠随机分为6组（每组10只）。各给药组按设定时间和剂量分别单独或联合腹腔注射相应药物，对照组给予相应容积的生理盐水。给药后不同时间点制备大脑中动脉栓塞一再灌注（MCAO）模型，栓塞2h后恢复灌注至72h。分别在再灌注24h、72h进行神经功能评分（NBS），72h评分后取脑行2，3，5-氯化三苯四唑（Trc）染色计算脑梗死容积百分比。结果单独使用人参皂甙Rd及人参皂甙Rd与川芎嗪和（或）葛根素联合使用均能显著改善大鼠MCAO损伤的NBS，减少脑梗死容积，各给药组间相比均无统计学差异。结论单独使用人参皂甙Rd和人参皂甙Rd与川芎嗪和（或）葛根素联合使用均具有明显的脑保护效应，但联合用药组没有表现出强于单独给药组的协同保护效应。

英文摘要：

Objective To investigate the neuroprotective effect of giusenaside Rd and the combined application of ginsenoside Rd with tetramethylpyrazine and （or） puerarin in rats and to compare the difference of neuroprotective effect between combined application of ginsenoside Rd with puerarin and （or） tetramethylpyrazine and ginseneside Rd alone. Methods Sixty male SD rats weighed 280 - 320 g were randomly assigned into 6 groups （n = 10 in each group）. Drugs were respectively infused intraperitoneally by single or combined method according to grouping protocol. The control group received infusions of normal saline. Rats were subjected to the right middle cerebral artery occlusion （MCAO） for 2 h, followed by reperfusion for 72 h. The neurological behavior scores （NBS） were assessed at 24 and 72 h after reperfusion. The percen｜age of infarct volume was determined at 72 h after reperfusion. Results Either ginsenoside Rd or the combined application of ginsenoside Rd with tetranrethylpyrazine and （or） puerarin could significantly improve the NBS and reduce the infarct volume after reperfusion as compared to the control group （P 〈 0.05）. No significant difference was fotmd in NBS and infarct volume among different drug administration groups. Concltt~＇ion Gin~noside Rd and the combined application of ginsenoside Rd with tetramethylpyrazine and （or） puerafin have similar neuroprotective effects. The combined application of ginsenoside Rd with tetramethylpyrazine and （or） puerarin do not generate synergistic effect as ginsenoside Rd alone.