中文摘要：

目的：研究StarD7以及Wnt/β-catenin信号通路促进睾酮合成的内在机制,探索睾酮合成的调控新途径。方法：用1 nmol/L的Annexin 5处理大鼠原代Leydig细胞24 h,化学发光法检测睾酮含量,利用RT-PCR和Westernblot方法分析StarD7和β-catenin在mRNA和蛋白水平上的表达变化,并利用免疫荧光法对β-catenin进行定位。结果：在Annexin 5的刺激作用下,与对照组相比,睾酮合成水平明显增加了176%[（7.83±0.32）vs.（21.6±1.1）,P〈0.05];在Annexin 5作用下,与对照组相比,StarD7在mRNA水平表达增加55%[（1.12±0.08）vs.（1.74±0.11）,P〈0.05],β-catenin表达增加48%[（1.15±0.08）vs.（1.70±0.05）,P〈0.05]。在蛋白水平上,与对照组相比,StarD7增加42%[（1.06±0.09）vs.（1.51±0.07,P〈0.05）],β-catenin增加55%[（1.02±0.01）vs.（1.58±0.02）,P〈0.05],此外在Annexin 5作用下,β-catenin有入核积聚的趋势。结论：在Annexin 5作用下,StarD7和β-catenin的表达均有显著增加,且β-catenin有入核积聚的趋势,提示StarD7和β-catenin对Annexin 5刺激Leydig细胞睾酮的合成均有调控作用,该过程可能是通过激活Wnt/β-catenin信号通路,从而促进StarD7表达上升,最终导致睾酮合成增加。

英文摘要：

Objective：This research aims to study the internal mechanism that promotes the testosterone synthesis by StarD7 and Wnt/β-catenin, and explores a new regulatory pathway of testosterone synthesis. Methods ： After treated with 1 nmol/L Annexin 5 for 24 h, the culture media were collected for testosterone measurement by chemiluminescence assay. The expressions of StarD7 and β-catenin at mRNA and protein levels were detected by RT-PCR and western blot respectively. The cellular location of β-catenin was identified by immunofluorescence. Results： Comparing with the control groups, under the treatment with Annexin 5, the level of testosterone raised 176% [ （7.83 ±0.32）vs. （21.6 ± 1.1 ）, P 〈0.05 ], StarD7 mRNA in the experimental groups increased 55% [ （ 1. 12 ± 0.08 ） vs. （ 1.74 -＋ 0. 11 ）, P 〈 0.05 ], and β-catenin mRNA increased 48% [ （ 1.15 ± 0.08） vs. （ 1.70 ± 0.05 ）, P 〈 0.05 ]. At the level of protein, the expression of StarD7 in the experimental groups increased 42% [ （ 1.06 ±0.09）vs. （ 1.51 ± 0.07）, P 〈 0.05 ], and β-catenin increased 55% [ （ 1.02 ± 0.01 ） vs. （ 1.58 ± 0.02）, P 〈 0.05 ]. Immunofluorescence identified that β-catenin was accumulation in the nuclear of the rat Leydig cells in the experiment groups cultured with Annexin 5. Conclusion： StarD7 and β-catenin have both increased significantly at the mRNA and protein levels under treatment with the Annexin 5, and β-catenin were accumulation in the nuclear of the rat Leydig cells. It suggests that StarD7 and β-catenin both regulate the effect of Annexin 5 in testosterone production of rat Leydig cells. This regulati Wnt/β-catenin signal pathway, then increase the expression of the StarD7, eventually of the testosterone secretion in rat Leydig cells. on may active the raise the progress of the testosterone secretion in rat Leydig cells.