中文摘要：

目的检测急性胰腺炎（AP）患者血清miR-216a的表达水平,探讨其作为AP诊断及预后监测指标的临床应用价值。方法实时荧光定量PCR（qRT-PCR）检测80例轻症急性胰腺炎（MAP）、80例重症急性胰腺炎（SAP）患者及74例健康人对照血清miR-216a的表达水平。全自动生化免疫分析仪检测各组淀粉酶（AMY）、脂肪酶（LPS）、Ca2＋、葡萄糖（Glu）、红细胞比容（HCT）、三酰甘油（TG）、总胆固醇（TC）和C反应蛋白（CRP）等指标。ROC曲线及相关性分析评估miR-216a对AP患者的临床应用价值。结果与健康人对照组[（11.12×10^（-5）（5.83×10^（-5）,19.12×10^（-5））]相比,AP患者血清miR-216a的水平[38.49×10^（-5）（24.05×10^（-5）,62.02×10^（-5））]明显升高（U=-9.10,P〈0.01）。但MAP与SAP患者血清miR-216a水平[分别为36.46×10^（-5）（22.29×10^（-5）,55.80×10^（-5））,40.44×10^（-5）（25.84×10^（-5）,65.48×10～（-5））]差异无统计学意义（U=-0.96,P〉0.05）。miR-216a用于鉴别对照组和AP患者的ROC曲线下面积（AUCROC）为0.870（95%CI：0.825～0.915）,cut-off值为0.61;用于鉴别对照组和MAP患者的AUCROC为0.865（95%CI：0.808～0.921）,cut-off值为0.59;用于鉴别对照组与SAP患者的AUCROC为0.876（95%CI：0.822～0.930）,cut-off值为0.66。AP患者治疗好转后,其血清miR-216a的表达水平由[41.88×10^（-5）（24.24×10^（-5）,64.44×10^（-5））]下降至[20.58×10^（-5）（11.01×10^（-5）,41.91×10^（-5））],差异有统计学意义（U=5.24,P〈0.01）。相关性分析结果显示,AP患者血清miR-216a的表达水平与CRP呈正相关（r=0.215 P=0.006）。结论 AP患者血清miR-216a水平明显升高,可用于AP辅助诊断及预后监测。

英文摘要：

Objective To detect altered levels and clinical significance of serum miR-216 a and prognosis monitoring in acute pancreatitis（ AP） patients. Methods Serum miR-216 a levels were determined by quantitative reverse-transcription PCR（ qRT-PCR） assay among 80 mild acute pancreatitis（ MAP） patients,80 severe acute pancreatitis（ SAP） patients and 74 healthy controls. And amylase（ AMY）,lipase（ LPS）,Ca2 ＋,glucose（ Glu）,hematocrit（ HCT）,triglyceride（ TG）,total cholesterol（ TC） and C reactive protein（ CRP） were measured by biochemical analyzer. The clinical usefulness of miR-216 a for AP patients was assessed by ROC curve analysis and correlation analysis. Results Compared with healthy controls（ 11. 12 × 10^（-5）[5. 83 × 10^（-5）,19. 12 × 10^（-5）],the serum miR-216 a levels were significantly increased in AP patients（ 38. 49 × 10^（-5）[24. 05 × 10^（-5）,62. 02 × 10^（-5）],（ U =-9. 10,P〈0. 01）.The serum miR-216 a levels in MAP and SAP patients were（ 36. 46 × 10^（-5）[22. 29 × 10^（-5）,55. 80 × 10^（-5）]vs 40. 44 × 10^（-5）[25. 84 × 10^（-5）,65. 48 × 10^（-5）]）,there was no significant difference between MAP and SAP patients（ U =-0. 96,P〈0. 05）. The areas under ROC curve（ AUCROC） of miR-216 a for differential healthy controls and AP patients was 0. 870（ 95% CI： 0. 825-0. 915）,cut-off value is0. 61. AUCROCof miR-216 a for differential healthy controls and MAP patients was 0. 865（ 95% CI： 0. 808-0. 921）,cut-off value is0. 59. And AUCROCof miR-216 a for differential healthy controls and SAP patients was 0. 876（ 95% CI： 0. 822-0. 930）,cut-off value is0. 66. Moreover,after the clinical improvement of the patients,the levels of serum miR-216 a were significantly lowered from（ 41. 88 ×10^（-5）[24. 24 × 10^（-5）,64. 44 × 10^（-5）]） to（ 20. 58 × 10^（-5）[11. 01 × 10^（-5）,41. 91 × 10^（-5）]）,the differences was significant（ U = 5. 24,P〈0. 01）. Correlation analys