在这研究,复杂 Mg (Sal_2trien ) 第一次被综合,与 CT, DNA 被紫外系列,荧光系列,粘性和 CV (循环伏安法) 的方法学习的有约束力的模式。在建筑群与 CTDNA 行动了以后,紫外系列的 Abs 显然升起了,这被发现;EB-DNA 的荧光紧张没被改变;粘性减少了。循环伏安法的决心证明 DNA 做了 MgL “ sformal 潜力否定地变。Scatchard 阴谋证明到 EB 的建筑群的有约束力的模式的增加与到 DNA 的 EB 相比是 uncompetitive 抑制。有 CT DNA 的建筑群的有约束力的模式因此是稳定电的绑定。然后,有 pBR322was 的建筑群的相互作用由凝胶电泳的方法学习了。结果出现了建筑群能 cleavepBR322 DNA 有效地。
In this study the complex Mg(Sal2trien) was synthesized for the first time, the binding mode of which with CT DNA was studied by the methods of UV spectra, fluorescence spectra, viscosity and CV (cyclic voltammetry). It was found that after the complex acted with CT DNA, the Abs of UV spectra rose obviously; the fluorescence intensity of EB-DNA was almost not changed; viscosity decreased. Determination of cyclic voltammetry showed that DNA made the MgL's formal potential negatively shift. Scatchard plot showed that the addition of the binding mode of the complex to EB was uncompetitive inhibition compared with EB to DNA. So the binding mode of the complex with CT DNA was stable-electricity binding. Then the interaction of the complex with pBR322 was studied by the method of gel electrophoresis. The result showed that the complex could cleave pBR322 DNA effectively.