中文摘要：

目的: 很多条证据证明进血浆房间的 B 淋巴细胞的区别在豺狼座致病起一个重要作用。在这研究，我们调查了怎么泼尼松，为自体免疫的疾病的古典治疗学的药，在 MRL/MpSlac-lpr mice.Methods 的调整血浆房间区别: MRL/lpr 老鼠与泼尼松被对待(2.5 或 5 mg·； kg ⁻¹·d⁻¹, ig ) 为 13 个星期，和 proteinuria，层次和幸存时间被监视。在老鼠是以后， euthanized，血样品，怒气和胸腺是镇定的。anti-dsDNA 抗体，反原子的抗体， IL-21，和 IL-10 的浆液层次用 ELISA 工具包被检测。脾的 B 和 T 淋巴细胞的子集与流动 cytometry 被确定。抄写因素 Blimp-1 和 Bcl-6 表示用 qPCR 和西方的 blot.Results 被决定: 泼尼松治疗 dose-dependently 与减少的 proteinuria 层次，延长幸存时间，反原子的抗体铺平的减少的浆液，和减少的怒气和胸腺索引在 MRL/lpr 老鼠稀释了豺狼座症状。泼尼松处理显著地也减少了血浆房间和血浆房间先锋的提高的百分比，减少百分比激活 T 房间，并且增加了 CD4 ⁺ CD62L ⁺ 房间，证明在豺狼座症状的减少的反原子的抗体和改进与减少的血浆房间被联系。而且，泼尼松处理减少了浆液 IL-21 和 IL-10 层次并且在 MRL/lpr mice.Conclusion 减少了脾的 Blimp-1 和 Bcl-6 (为血浆房间区别的二个关键规章的因素)的表达式:泼尼松处理在 MRL/lpr 鼠标限制 B 淋巴细胞区别进血浆房间，它可以与 IL-21 生产的抑制和在 Blimp-1 和 Bcl-6 之间的平衡的恢复被相关。

英文摘要：

Aim： A number of evidence shows that the differentiation of B lymphocytes into plasma cells plays an important role in lupus pathogenesis. In this study we investigated how prednisone, a classical therapeutic drug for autoimmune diseases, regulated plasma cell differentiation in MRL/MpSlac-lpr mice. Methods： MRL/Ipr mice were treated with prednisone （2.5 or 5 mg·kg^-1·d^-1, ig） for 13 weeks, and the proteinuria levels and survival times were monitored. After the mice were euthanized, blood sample, spleen and thymus were collected. The serum levels of anti-dsDNA antibody, anti-nuclear antibody, IL-21, and IL-10 were detected using ELISA kits. Subsets of splenic B and T lymphocytes were quantified with flow cytometry. Transcription factor Blimp-1 and Bcl-6 expression was determined using qPCR and Western blot. Results： Prednisone treatment dose-dependently attenuated the lupus symptoms in MRL/Ipr mice with decreased proteinuria levels, prolonged survival times, decreased serum anti-nuclear antibody levels, and reduced spleen and thymus indices. Prednisone treatment also significantly decreased the elevated percentages of plasma cells and plasma cell precursors, decreased the percentages of activated T cells, and increased the frequency of CD4^＋CD62L^＋ cells, demonstrated that decreased anti-nuclear antibodies and improvements in lupus symptoms were associated with decreased plasma cells. Furthermore, prednisone treatment decreased serum IL-21 and IL-10 levels and reduced the expression of splenic Blimp-1 and Bcl-6 （two key regulatory factors for plasma cell differentiation） in MRL/Ipr mice. Conclusion： Prednisone treatment restricts B lymphocyte differentiation into plasma cells in MRL/Ipr mice, which may be correlated with the inhibition of IL-21 production and the restoration of the balance between Blimp-1 and Bcl-6.