中文摘要：

肿瘤微环境指肿瘤细胞发生及转移所处的复杂三维环境。传统研究多让细胞生长在二维培养瓶或培养皿中，缺少肿瘤转移过程中复杂的细胞．细胞和细胞．基质问的动态联系，难以反映肿瘤组织的自身特点以及阐明肿瘤细胞转移的机制，也限制了有效药物的准确检测。为阐明原发性肿瘤微环境的特点，多种生物模拟的三维工程化肿瘤模型已被用于实验和临床研究，即将肿瘤基质细胞、基质组分、生物化学和生物物理学信号整合于同一个时空系统。本文总结了典型三维工程化肿瘤的构建及应用。

英文摘要：

Tumor microenvironment is a complex three-dimensional environment involved in the occurrence and metastasis of tumor cells. Ceils are mostly grown in two-dimensional culture plate or petri dish in traditional in vitro researches, which is lack of the complex and dynamic cell-cell communication and cell-ma- trix interaction during cancer metastasis. It is difficult to reflect the essential feature of tumor tissue, clarify the mechanism of cancer metastasis fundamentally, and limit the applicability to accurately test the pharmacolog- ical action. In order to illustrate the feature of primary tumor microenvironment, various biomimetically engi- neered three-dimensional tumor models have been developed in experimental and clinical researches, in which tumor stromal cells, relevant matrix components, and biochemical and biophysical cues are incorporated into one spatially and temporally integrated system. In this study, the construction and application of diversified engineered three-dimensional tumor are reviewed.