中文摘要：

常规轻显微镜学的决定为 subcelluar 研究是不够的。各种各样的超级决定的成像技术的发明打破衍射障碍并且向纳米规模推决定限制。这里，我们集中于依靠随机的激活和单个分子的精确本地化的超级决定的显微镜学的一个范畴。多种荧光灯有不同特征的探针被开发了完成超级决定的成像。与柔韧的本地化算法的实现，另外，途径的这个家庭被扩展了到多色，三维、实时的房间成像，它在生物研究提供有希望的前景。

英文摘要：

The resolution of conventional light microscopy is insufficient for subcelluar studies. The invention of various super-resolution imaging techniques breaks the diffraction barrier and pushes the resolution limit towards the nanometer scale. Here, we focus on a category of super-resolution microscopy that relies on the stochastic activation and precise localization of single molecules. A diversity of fluorescent probes with different characteristics has been developed to achieve super-resolution imaging. In addition, with the implementation of robust localization algorithms, this family of approaches has been expanded to multi-color, three-dimensional and live cell imaging, which provides a promising prospect in biological research.