中文摘要：

Technological advances are important for innovative biological research. Development of molecular tools for DNA manipulation, such as zinc finger nucleases(ZFNs), transcription activator-like effector nucleases(TALENs), and the clustered regularly-interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas), has revolutionized genome editing. These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases. In the last few years, substantial progress has been made in CRISPR/Cas technology, including technical improvements and wide application in many model systems. This review describes recent advancements in genome editing with a particular focus on CRISPR/Cas, covering the underlying principles, technological optimization, and its application in zebrafish and other model organisms, disease modeling, and gene therapy used for personalized medicine.

英文摘要：

Technological advances are important for innovative biological research. Development of molecular tools for DNA manipulation, such as zinc finger nucleases （ZFNs）, transcription activator-like effector nucleases （TALENs）, and the clustered regularly-interspaced short palin- dromic repeat （CR1SPR）/CRISPR-associated （Cas）, has revolutionized genome editing. These approaches can be used to develop potential therapeutic strategies to effectively treat heritable dis- eases. In the last few years, substantial progress has been made in CRISPR/Cas technology, includ- ing technical improvements and wide application in many model systems. This review describes recent advancements in gennme editing with a particular focus on CRISPR/Cas, covering the under- lying principles, technological optimization, and its application in zehrafish and other model organ- isms, disease modeling, and gene therapy used for personalized medicine.