以正交试验方法,取得了蜂胶CO2超临界萃取最佳提取条件:即萃取温度为46℃、萃取压力为25MPa、分离1温度为50℃、分离1压力为7MPa、萃取时间为3h;发现加入5%~10%乙醇作为萃取的携带剂,有利于提高蜂胶超临界萃取率;同时发现CO2超临界萃取是一种去除蜂胶原料中铅的有效方法.但不能富集蜂胶中黄酮类化合物。
By using method of statistics, the best factors of CO2 supercritical extraction have been got. The result shows : the temperature and pressure of extraction are 46 ℃ and 25 MPa, the temperature and pressure of separation 1 are 50 ℃ and 7 MPa, the time of extraction is 3 h. We found when the 5% - 10% alcohol as reagent was put in propolis, it can increase the rate of CO2 supercritical extraction of propolis. Simultaneity, the method of CO2 supercritical extraction can eliminate plumbum in propolis, but it can not enrich flavonoid in propolis.