中文摘要：

目的观察膜联蛋白5（annexin5）对人精子线粒体功能的影响。方法收集38例少弱精症患者精液标本。精子与an-nexin5共温育,用罗丹明（Rh123）和碘化吡啶（PI）染色并用流式细胞术检测人精子线粒体功能。结果添加annexin5组与对照组相比,线粒体功能良好的活精子百分率[（38.77±9.20）%vs（26.12±10.09）%,P〈0.05]和线粒体荧光值[（509.72±45.02）vs（452.88±39.39）,P〈0.05]均有显著性升高。结论体外添加annexin5能增强精子线粒体的活性。

英文摘要：

Objective To study the effects of annexin 5 on the function of human sperm mitochondria in vitro.Methods Semen specimens of 38 patients were exposed to annexin 5 in vitro,and the effects of annexin 5 on the function of human sperm mitochondia were investigated with flow cytometry after staining with rhodamine 123（Rh123） and propidium iodide（PI）.Results The percentage of sperms with normal mitochondrial function in annexin 5 group[（38.77±9.20）%] increased significantly compared with the control group [（26.12±10.09）%,P0.05].The fluorescent value of sperm mitochondria（509.72±45.02） increased significantly compared with the control group（452.88±39.39）,P0.05.Conclusion Annexin 5 may improve human sperm mitochondrial activity in vitro.