中文摘要：

目的探讨内皮祖细胞（EPC）输注对异基因造血干细胞移植（allo—HSCT）小鼠造血重建的影响。方法白消安（BU）／环磷酰胺（CY）预处理后建立C57BL／6供BABL／c小鼠allo—HSCT模型。受鼠随机分为4组：未处理组、BU／CY预处理组、单纯移植组和EPC联合移植组。移植后动态观察受鼠骨髓病理变化，MECA32抗体免疫组化染色观察骨髓窦内皮和微血管的变化；流式细胞术分析骨髓内干、祖细胞比例及血清细胞因子；并行外周血血细胞计数。结果①联合EPC输注组小鼠骨髓中造血组织、骨髓窦内皮及微血管的损伤较单纯移植组轻。②予EPC输注后，受鼠骨髓中造血干细胞比例在移植后21天（＋21d）内明显增高，联合移植组小鼠HSC于＋14d达高峰，明显高于单纯移植组（0．1743对0．0787）（P〈0．05），骨髓造血祖细胞移植后持续增高，＋21d时联合EPC组显著高于单纯移植组（0．4550对0．3905）（P〈0．05）。③联合移植组外周血白细胞计数早期始终高于单纯移植组，＋14d达到高值，（0．74×10^9／L对0．47×10^9／L）（P〈0．05），外周血血小板计数于＋14d升至最高，联合EPC移植组显著高于单纯移植组（1228．9×10^9／L对977．12×10^9／L）（P〈0．05）。结论allo—HSCT联合EPC输注可促进小鼠移植后的造血重建。

英文摘要：

Objective To examine the effects of endothelial progenitor cell （EPC） on hematopoietic reconsititution in allogeneic hematopoietic stem cell transplantation （allo-HSCT） mouse model. Methods Allo-HSCT mouse model was established with condition of BU/CY, in which C57BL/6 （H-2b） and BABL/c （H-2d） mice were used as donors and recipients respectively. Recipients were randomly divided into 4 groups： untreated group, BU/CY condition group, bone marrow transplantation （BMT） group and transplan- tation of BM cells combined with EPCs （ combined transplantation） group. The pathological changes of BM cells following transplantation were dynamically observed. Changes of BM sinusoidal endothelium and anglo- genesis were observed by MECA-32 antibody immunohistochemical staining. The proportion of intramedullary stem and progenitor cells and serum cytokines were analyzed by flow cytometry. The numbers of peripheral blood cells were also counted. Results①Injuries of BM hematopoietic tissue, sinusoidal endothelium and vascular were less severe in combined transplantation group than of BMT group. ②EPC infusion significantly increased BM hematopoietic stem ceils 21 days after transplantation. The percentage of BM hematopoietic stem cells in combined transplantation group peaked on day ＋ 14, which was higher than of BMT group （0. 1743 vs 0. 0787 ） （P 〈 0.05 ）. The continuously increased percentage of BM hematopoietic progenitor cells in combined transplantation group was significantly higher than in BMT group on day ＋21 （0.4550 vs 0. 3905 ） （ P 〈 0.05 ）.③The number of peripheral white blood cells in combined transplantation group was always higher than of BMT group, which reached the peak on day ＋ 14 （0.74±10^9/L to 0.47×10^9/L） （P 〈 0.05）. The peak number of peripheral blood platelets on day ＋ 14 in combined transplantation group was significantly higher than of group BMT （ 1228.9 × 10^9/L to 977.12×10^9/L） （P 〈 0.05 ）. Conclusion Allo-HSCT combined