中文摘要：

目的研究香蒲新苷对人脐动脉血管平滑肌细胞增殖的影响.方法①采用组织贴块法原代培养人脐动脉平滑肌细胞（ HUASMC）,并用α-actin抗原检测进行鉴定,3～4代细胞用于实验.②香蒲新苷以1.00×10^-5mol/L为最大浓度,依次倍比稀释,共11个浓度,并设空白对照组,分别刺激24、48、72 h,采用MTT比色法检测其对 HUASMC有无毒性.③香蒲新苷以1.00×10^-6mol/L为最大浓度,依次按1/4等比稀释,共6个浓度,并设空白对照组,分别刺激24、48、72 h,采用MTT比色法观察其对 HUASMC增殖的影响.结果①镜下观察细胞染色均为阳性.②香蒲新苷在9.77×10^-9～1.00×10^-5mol/L的浓度范围内,刺激24、48、72 h,对体外培养的 HUASMC均无明显的毒性.③香蒲新苷在9.77×10^-10～1.00×10^-6 mol/L的浓度范围内,刺激24 h或48 h,对 HUASMC的增殖均无明显的抑制作用;而在作用72 h后,在1.56×10^-8～2.5×10^-7mol/L之间,对 HUASMC的增殖有明显的抑制作用,其作用强度随剂量增加略有增强,但无显著差异,抑制率达9.91%～14.99%.结论香蒲新苷具有抑制血管平滑肌细胞增殖的作用,其有效剂量范围较广,但起效缓慢.

英文摘要：

Objective To study the influence of typhaneoside on the proliferation of human umbilical arterial smooth muscle cells （HUASMC）. Methods ① HUASMC were primary cultured with tissuesticking method and determined by α-actin detection. The third to fourth generations of cells were put into the experiment. ② The highest concentration of typhaneoside was 1.00 × 10^-5 mol/L and then diluted in muhiple proportions into 11 concentration groups, and a blank control group was established. After the stimulations were given to different groups for 24,48 and 72 hours, the toxicity of typhaneoside were detected with MTT assay. ③ The highest concentration of typhaneoside was 1.00 × 10^ -6 mol/L and then diluted in 1/4 isobaric proportion into 6 concentration groups, and a blank control group was established. After the stimulations were given to different groups for 24, 48 and 72 hours, the influence of typhaneoside on the proliferation of HUASMC was observed with MTT assay. Results ① The cellular staining was positive under the microscope. ② Typhaneoside was not toxic to HUASMC in the concentration ranges from 9. 77 × 10^-9 mol/L to 1.00 × 10^-5 mol/L after 24, 48 and 72 hours.③ Typhaneoside did not inhibit the proliferation of HUASMC in the concentration ranges of 9.77 × 10^-10 mol/L to 1.00 × 10^-6 mol/L after 24 or 48 hours, but inhibited significantly the proliferation of HUASMC after 72 hours in the concentration ranges of 1.56 × 10^-8 mol/L to 2.5 ×10^-7 mol/L. The effect was increased along with the dose increase but there was no significant difference. The inhibitory rate reached 9.91% to 14.99%. Conclusion Typhaneoside has an inhibitory effect on the proliferation of HUASMC with a wide range of effective dose but the effect is started slowly.