利用石蜡切片以及半薄切片法对狭叶柴胡的胚及胚乳的发育进行了研究,并对采收期果实中胚的发育情况进行了统计。结果表明:(1)狭叶柴胡胚的发育属于茄型;胚乳核的发育早于合子的分裂,胚乳的发育属于核型。(2)于采收期对狭叶柴胡果实的胚发育阶段观察发现,狭叶柴胡采收期有26%的果实处于球形胚时期,29.6%的果实处于早期心形胚时期,37.4%的果实处于后期心形胚时期,仅有7%果实处于鱼雷胚时期,所有被检测的果实中未发现有双子叶时期胚出现。研究表明,狭叶柴胡采收期果实存在严重的形态后熟现象,是导致在其栽培过程中出现发芽率低和出苗不整齐现象的主要原因。
The development of embryo and endosperm of Bupleurum scorzonerifolium was studied by means of paraffin section and semi-thin section,and the developmental degree of seed at collected stage was counted. (1)The development of B, scorzonerifolium embryo belongs to the solanad type; the division of endosperm nucleus is earlier than zygote,and the development of endosperm belongs to the karyotype. (2) Statistics study on embryos in fruits at being collected stages showed that the embryo rate of globular em- bryo,early heart-shape embryo,late heart-shape embryowas at 26% ,29.6%and 37.4%respectively. How- ever, merely 7 % was determined for fish torpedo embryo, and dicotyledonous embryo was rarely detected in the experimental fruits. Therefore, the fruits of B. scorzonerifoliurn were characterized by serious post- maturation phenomenon,which is the primary cause resulting in low germination percentage and irregular emergency phenomena about B. scorzonerifolium during its cultivation process.