中文摘要：

目的：构建一种具有超亲水、多尺度有序结构的种植体表面。方法：经砂纸打磨的钛片先喷砂、硝酸/氢氟酸处理,再用盐酸/硫酸预酸蚀,最后用双氧水/硫酸处理获得多尺度有序结构的表面。用扫描电镜观察表面形貌;接触角表征该表面的亲水性;X射线衍射（XRD）分析表面物相;体外成骨前体细胞培养观察该表面对细胞粘附、增殖和分化的影响。结果：扫描电镜显示,种植体表面具有微纳复合结构;XRD图谱显示二次酸蚀处理前后,表面的成分无明显改变;但是,接触角则下降到0°。体外生物学评价表明：该表面有利于细胞的粘附,促进细胞的增殖和分化。1 h、3 h和72 h实验组每个钛片上细胞的DNA含量分别为（710±78）ng、（1 398±102）ng和（2 543±113）ng,对照组相应时间点的DNA含量分别为（605±79）ng、（1 045±125）ng和（2 138±97）ng,3个时间点实验组的DNA含量均高于对照组（P〈0.05）。细胞内的ALP活性检测显示：实验组4 d、7 d和14 d对硝基酚生成速率分别为（0.09889±0.00417）nmol/（ng.h）、（0.15008±0.00406）nmol/（ng.h）和（0.27862±0.00305）nmol/（ng.h）,对照组相应时间点分别为（0.07862±0.00406）nmol/（ng.h）、（0.12256±0.00399）nmol/（ng.h）和（0.22641±0.0142）nmol/（ng.h）,3个时间点实验组ALP活性显著高于对照组（P〈0.05）。实验组和对照组第14天培养液中骨钙素（OC）含量分别为（975±110）ng/mg和（545±69）ng/mg,差异具有统计学意义（P〈0.05）。结论：本研究成功获得了一种超亲水的微纳复合结构表面,该表面能进一步促进成骨前体细胞的生长行为。

英文摘要：

Objective： To construct a multiple-scale organized implant surface with super- hydrophilicity. Methods： The SiC paper polished titanium disc was sandblasted and treated with HF/ HNO3 and HC1/H2 SO4, then acid-etched with H2 SO4/H202. The physicochemical properties of the surfaces were characterized by scanning electron microscope, static state contact angle and X-ray diffraction. MC3T3-E1 cells were used to evaluate the effects of the surface on the cell adhesion, proliferation and differentiation. Results： The acid-etching process with a mixture of H2 SO4/H2 02 superimposed the nano-scale structure on the micro-scale texture. The multiple-scale implant surface promoted its hydrophilicity and was more favorable to the responses of osteoprogenitor cells, characterized by increased DNA content, enhanced ALP activity and promoted OC production. Conclusion： A multiple-scale implant surface with super-hydrophilicity has been constructed in this study, which facilitates cell proliferation and adhesion.