中文摘要：

采用高效液相色谱-电感耦合等离子体质谱（HPLC-ICP-MS）联用技术对染砷后Chang肝细胞中的砷代谢产物进行分析。通过参考文献及相关实验推测出两种未知砷代谢产物,并采用标准合成加入法证明其中一种未知砷代谢产物为一甲基亚胂酸（MMAⅢ）。定量分析结果显示,随着染砷浓度的增大,细胞中的一甲基砷、二甲基砷以及无机砷含量均呈现增大趋势,而甲基化率却呈现减小趋势,这说明高浓度砷对细胞甲基化能力有抑制作用。

英文摘要：

Hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry（HPLC-ICP-MS） was applied to the determination of arsenic metabolites in Chang liver cells after arsenic incubation.Two unknown arsenicals were found,one of which was speculated as methylarsonous acid（MMAⅢ）,and further characterized using standard addition method using synthesized MMA Ⅲ standard.Quantitative analysis results showed that monomethylarsenicals,dimethylarsenicals and inorganic arsenicals in hepatocytes after arsenic incubation were increasing with the increase of initial incubated arsenic concentration while the methylation rate decreasing with the increase of initial incubated arsenic concentration,which indicated that high concentration of arsenic has the inhibition to the ability of arsenic methylation of Chang liver cells.