实验研究了凝胶材料制备的光合细菌包埋颗粒构成的光生物制氢填充床在连续操作条件下底物浓度、光照强度以及进口流量等参数影响下的产氢和降解有机物的性能。实验结果表明:填充床产氢速率和底物降解速率随进口葡萄糖浓度的增加而增大,且达到最佳的进口底物浓度后填充床产氢和底物降解速率呈下降趋势,表明光合细菌代谢底物为产氢提供还原力氢;光照强度低于光能饱和度时,随着光照强度的增大,产氢速率和底物降解速率呈递增趋势,光照强度超过光能饱和度则对填充床光合产氢和底物消耗产生明显抑制作用;进口流量较低时,随进口流量的增大,填充床产氢和底物降解速率明显增大,进口流量较高时,填充床产氢和底物降解速率趋于相对稳定。
Effect of substrate concentration, light intensity and influent flux on characteristics of hydrogen production and biodegradation of substrate by packed bed which filled with particles entrapped photosynthetic bacteria by sodium alginate-PVA gel was investigated in continuous cultures. It revealed that both the hydrogen producing rate and biodegradation rate increased firstly up to a maximum and then dropped off with the increase in glucose concentration, which showed that reduced hydrogen came from substrate such as glucose. Similarly, there existed corresponding saturation of light intensity to hydrogen producing and biodegradation of substrate as the rate of hydrogen production and substrate biodegradation in packed bed increased with light intensity lower than light saturation increasing, contrarily, hydrogen production and substrate biodegradation would be compressed by light intensity higher than light saturation. Meanwhile, the rise of low flux leaded to a significant enhancement of hydrogen production and biodegradation. However, the rate of hydrogen production and substrate biodegradation became steady relatively at high inlet flux.