中文摘要：

目的：研究复制缺陷型腺病毒（replication deficient adenoviral vectors，AdVec）介导外源基因增强型绿色荧光蛋白（enhanced green fluorescent protein，EGFP）感染体外培养的神经干细胞（neural stem cells，NSCs）表达的可行性及感染效率，探讨EGFP作为NSCs示踪剂的可行性。方法：体外原代培养、扩增大鼠NSCs，扩增、纯化AdVec—EGFP病毒液，以AdVec-EGFP病毒液感染NSCs，荧光显微镜观察AdVec-EGFP在NSCs中的表达和转染效率，免疫细胞化学鉴定重组子。结果：EGFP报告基因AdVec-EGFP高效感染NSCs，转染后6h荧光蛋自开始表达，48h后达高峰，感染率为（76±2）％，且NSCs的生物学性状没有发生变化。结论：AdVec-EGFP具有较高的介导外源基因表达于NSCs的效率，是NSCs体内、体外研究较理想的示踪剂。

英文摘要：

Objective：To study the feasibility and infection efficiency of replication deficient adenoviral vectors （AdVec） mediated enhanced green fluorescent protein （EGFP） transduction to neural stem cells （NSCs）. Methods：Rat＇s NSCs were cultured in vitro, AdVec-EGFP virus were amplified and purified. NSCs were transduced with AdVec-EGFP virus and results were estimated with fluorescence microscope and immnocytochemistry. Results：Report gene EGFP could be high performed in infected NSCs; fluorescein started to be expressed 6 hours after trasfected, and reached peak time afer 48 hours. The infection rate was （76 ± 2）% ; the biological characters of NSCs had no changes. Conclusions：AdVec-EGFP has higher gene expression efficiency of mediating exogenous gene; it is a better labelled compound for NSCs investigation both in vivo and in vitro.